Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Xanthobacter autotrophicus Py2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
acetone | - |
Xanthobacter autotrophicus Py2 | |
0.122 | - |
ATP | - |
Xanthobacter autotrophicus Py2 | |
4.17 | - |
CO2 | cosubstrate bicarbonate | Xanthobacter autotrophicus Py2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Xanthobacter autotrophicus Py2 | |
additional information | addition of Fe2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+ or Ni2+ do not stimulate the enzyme activity above the maximal levels obtained in the presence of Mg2+ alone | Xanthobacter autotrophicus Py2 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19600 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
23000 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
68400 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
78300 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
78800 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
85300 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
353000 | - |
native molecular weight of enzyme complex | Xanthobacter autotrophicus Py2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus Py2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose chromatography, gel filtration with Sephacryl S-300 column, HiLoad Q-Sepharose column | Xanthobacter autotrophicus Py2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.094 | - |
usage of butanone | Xanthobacter autotrophicus Py2 |
0.225 | - |
usage of acetone at 30°C and pH 7.6 | Xanthobacter autotrophicus Py2 |
Storage Stability | Organism |
---|---|
4°C, pH 6.5-8.0, activity stable for several days | Xanthobacter autotrophicus Py2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetone + CO2 + ATP + H2O | - |
Xanthobacter autotrophicus Py2 | acetoacetate + AMP + phosphate | - |
? | |
butanone + CO2 + ATP + H2O | - |
Xanthobacter autotrophicus Py2 | 3-oxopentanoate + AMP + phosphate | - |
? | |
additional information | enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells | Xanthobacter autotrophicus Py2 | ? | - |
? | |
additional information | 2-pentanone, 3-pentanone, 2-hexanone and chloroacetone are substrates | Xanthobacter autotrophicus Py2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Xanthobacter autotrophicus Py2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Xanthobacter autotrophicus Py2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | obligate requirement | Xanthobacter autotrophicus Py2 |