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Literature summary for 6.4.1.5 extracted from
- Geranoyl-CoA carboxylase: a novel biotin-containing enzyme in plants (1999), Arch. Biochem. Biophys., 362, 12-21.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Avidin | complete inhibition, prevented by incubation of avidin with biotin prior to its addition | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0084 | - |
ATP | - |
Zea mays |  |
| 0.064 | - |
geranoyl-CoA | - |
Zea mays | |
| 0.58 | - |
HCO3- | - |
Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Zea mays | 9536 | - |
| plastid | 84% of GCCase activity is recovered in plastids | Daucus carota | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 122000 | - |
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + geranoyl-CoA + HCO3- | Zea mays | plays an important role in isoprenoid catabolism | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
| ATP + geranoyl-CoA + HCO3- | Glycine max | plays an important role in isoprenoid catabolism | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
| ATP + geranoyl-CoA + HCO3- | Daucus carota | plays an important role in isoprenoid catabolism | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
- |
- |
| Glycine max | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 184fold, from leaves | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities in different developing embryos, effect of cotyledon development and cell growth on GCCase activity | Daucus carota |
| additional information | - |
specific activities in several tissues of 4 and 15 days old seedlings | Glycine max |
| additional information | - |
specific activities in several tissues of 7 days old seedlings | Zea mays |
| 0.138 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + geranoyl-CoA + HCO3- | ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA | Zea mays | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | formation of gamma-carboxygeranoyl-CoA | ? | |
| ATP + geranoyl-CoA + HCO3- | ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA | Glycine max | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | formation of gamma-carboxygeranoyl-CoA | ? | |
| ATP + geranoyl-CoA + HCO3- | ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA | Daucus carota | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | formation of gamma-carboxygeranoyl-CoA | ? | |
| ATP + geranoyl-CoA + HCO3- | plays an important role in isoprenoid catabolism | Zea mays | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
| ATP + geranoyl-CoA + HCO3- | plays an important role in isoprenoid catabolism | Glycine max | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
| ATP + geranoyl-CoA + HCO3- | plays an important role in isoprenoid catabolism | Daucus carota | ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA | - |
? | |
| additional information | not: methylcrotonyl-CoA | Zea mays | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCCase | - |
Zea mays |
| GCCase | - |
Glycine max |
| GCCase | - |
Daucus carota |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
active over a broad pH-range | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | biotin-dependent enzyme | Zea mays | |
| biotin | biotin-dependent enzyme | Glycine max | |
| biotin | biotin-dependent enzyme | Daucus carota | |
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Release 2023.1 (January 2023)
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