Cloned (Comment) | Organism |
---|---|
MCCase subunits are co-expressed in Escherichia coli in the same plasmid and purified in one step by affinity chromatography using the LiuD-His tag protein interacting with the LiuB-S tag recombinant protein | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
crystal structures diffracted to 1.5 A resolution of the Pseudomonas aeruginosa MCC (PaMCC) holoenzyme are shown, alone and in complex with coenzyme A. The structures show that the architecture and overall shape of PaMCC are strikingly different when compared to propionyl-CoA carboxylase | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.168 | - |
3-methylcrotonoyl-CoA | Vmax: 430 nmol/mg/min, pH and temperature not specified in the publication | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
490000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I299 and Q9I297 | Q9I299, alpha-subunit and Q9I297, beta-subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas aeruginosa | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | co-expressed subunits form an (alpha-beta)4 complex that suffer a modification of its oligomerization | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
3-methylcrotonyl coenzyme-A carboxylase | - |
Pseudomonas aeruginosa |
liuB | encoding beta subunit of MCCase | Pseudomonas aeruginosa |
liuD | encoding alpha subunit of MCCase | Pseudomonas aeruginosa |
MCCase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Pseudomonas aeruginosa |