Activating Compound | Comment | Organism | Structure |
---|---|---|---|
biotin | biotin-dependent enzyme | Acidianus brierleyi | |
palmitoyl-CoA | slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) | Acidianus brierleyi |
Cloned (Comment) | Organism |
---|---|
- |
Acidianus brierleyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | above 2.0 mM | Acidianus brierleyi | |
citrate | 3 mM, more than 50% inhibition | Acidianus brierleyi | |
CoA | 1 mM, 60% inhibition of propionyl-CoA carboxylase | Acidianus brierleyi | |
DTT | 5-10 mM, 12% inhibition | Acidianus brierleyi | |
K+ | - |
Acidianus brierleyi | |
malonyl-CoA | 0.5 mM, 60% inhibition of propionyl-CoA carboxylase | Acidianus brierleyi | |
methylmalonyl-CoA | 0.5 mM, 60% inhibition of propionyl-CoA carboxylase | Acidianus brierleyi | |
Mg2+ | above 4.0 mM | Acidianus brierleyi | |
Mn2+ | above 2.0 mM | Acidianus brierleyi | |
Na+ | - |
Acidianus brierleyi | |
succinyl-CoA | 0.5 mM, 57% inhibition of propionyl-CoA carboxylase | Acidianus brierleyi | |
sulfate | 25 mM, more than 50% inhibition | Acidianus brierleyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
propanoyl-CoA | pH 7.5, 70°C | Acidianus brierleyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 26% of the maximum activity is observed when Co2+(10 to 15 mM) is used as a divalent cation | Acidianus brierleyi | |
Mg2+ | maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM | Acidianus brierleyi | |
Mn2+ | 1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion | Acidianus brierleyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE | Acidianus brierleyi |
59000 | - |
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE | Acidianus brierleyi |
62000 | - |
4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE | Acidianus brierleyi |
540000 | - |
- |
Acidianus brierleyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus brierleyi | Q877I4 and Q877I3 and Q877I5 | Q877I4: biotin carboxyl carrier protein of acetyl-CoA carboxylase, Q877I3: carboxyltransferase subunit of acetyl-CoA carboxylase, Q877I5: biotin carboxylase subunit of acetyl-CoA carboxylase | - |
Purification (Comment) | Organism |
---|---|
- |
Acidianus brierleyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.9 | - |
pH 7.5, 70°C, propionyl-CoA carboxylase activity | Acidianus brierleyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA | Acidianus brierleyi | ? | - |
? | |
ATP + propanoyl-CoA + HCO3- | the bifunctional enzyme also catalyzes the carboxylation of acetyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM) | Acidianus brierleyi | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
additional information | neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme | Acidianus brierleyi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE | Acidianus brierleyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Acidianus brierleyi |
70 | - |
assay at | Acidianus brierleyi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | 50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity | Acidianus brierleyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
2 h, 40% loss of activity | Acidianus brierleyi |
60 | - |
30 min, 50% loss of activity | Acidianus brierleyi |
70 | - |
30 min, 90% loss of activity | Acidianus brierleyi |
80 | - |
30 min, 95% loss of activity | Acidianus brierleyi |
90 | - |
10 min, complete loss of activity | Acidianus brierleyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.9 | at 70°C | Acidianus brierleyi |
7.5 | - |
assay at | Acidianus brierleyi |