Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A497V | polymorphism, 30% higher specific activity than wild-type enzyme | Homo sapiens |
E168K | mutant enzyme shows 32.3% of the specific activity of purified wild-type enzyme | Homo sapiens |
R165W | mutant enzyme shows 42.5% of the specific activity of purified wild-type enzyme | Homo sapiens |
R410W | mutant enzyme shows 42.61% of the specific activity of purified wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme E168K | Homo sapiens | |
0.33 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme R165W | Homo sapiens | |
0.41 | - |
propanoyl-CoA | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.54 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme R410W | Homo sapiens | |
0.59 | - |
propanoyl-CoA | 37°C, pH 8.0, mutant enzyme A497V | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
800000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.54 | - |
purified mutant enzyme E168K | Homo sapiens |
9.93 | - |
purified mutant enzyme R165W | Homo sapiens |
9.95 | - |
purified mutant enzyme R410W | Homo sapiens |
23.35 | - |
purified wild-type enzyme | Homo sapiens |
32.34 | - |
purified mutant enzyme A497V | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + propanoyl-CoA + HCO3- | - |
Homo sapiens | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | alpha6beta6 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PCC | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal denaturation is irreversible | Homo sapiens |
37 | - |
30 min, wild-type enzyme is stable, mutant enzymes R165W, E168K and R410W lose 30% of their activity | Homo sapiens |
47 | - |
30 min, wild-type enzyme is stable, mutant enzyme A497V loses 40% of its activity mutant enzymes R165W, E168K and R410W lose 85% of their activity | Homo sapiens |
58 | - |
the wild-type enzyme undergoes a cooperative two-state transition between the native and denatured states with a Tm of 57.6°C | Homo sapiens |