General Stability | Organism |
---|---|
limited proteolysis with trypsin results in slow time-dependent deactivation of the enzyme with preferential cleavage of the smaller subunit | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | - |
Homo sapiens | |
0.29 | - |
propanoyl-CoA | - |
Homo sapiens | |
1.2 | - |
butanoyl-CoA | - |
Homo sapiens | |
3 | - |
HCO3- | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates, Km: 9.4 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions | Homo sapiens |
72000 | - |
x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions | Homo sapiens |
540000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | at a rate 1.5% that obtained for propionyl-CoA | Homo sapiens | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + butanoyl-CoA + HCO3- | - |
Homo sapiens | ADP + phosphate + ? | - |
? | |
ATP + propanoyl-CoA + HCO3- | - |
Homo sapiens | ADP + phosphate + methylmalonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-50 | 37 | stable | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.8 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.6 | 7.2-8.8: maximal activity, 9.6: about 50% of maximal activity | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.4 | stable | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | biotin-containing enzyme | Homo sapiens | |
biotin | 4 mol of biotin bound per mol of enzyme | Homo sapiens |