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Literature summary for 6.4.1.3 extracted from

  • Kalousek, F.; Darigo, M.D.; Rosenberg, L.E.
    Isolation and characterization of propionyl-CoA carboxylase from normal human liver. Evidence for a protomeric tetramer of nonidentical subunits (1980), J. Biol. Chem., 255, 60-65.
    View publication on PubMed

General Stability

General Stability Organism
limited proteolysis with trypsin results in slow time-dependent deactivation of the enzyme with preferential cleavage of the smaller subunit Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
ATP
-
Homo sapiens
0.29
-
propanoyl-CoA
-
Homo sapiens
1.2
-
butanoyl-CoA
-
Homo sapiens
3
-
HCO3-
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates, Km: 9.4 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions Homo sapiens
72000
-
x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions Homo sapiens
540000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3- at a rate 1.5% that obtained for propionyl-CoA Homo sapiens ADP + phosphate + malonyl-CoA
-
?
ATP + butanoyl-CoA + HCO3-
-
Homo sapiens ADP + phosphate + ?
-
?
ATP + propanoyl-CoA + HCO3-
-
Homo sapiens ADP + phosphate + methylmalonyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 72000, alpha, + x * 56000, beta, SDS-PAGE under reducing conditions Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-50 37 stable Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 8.8
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.6 7.2-8.8: maximal activity, 9.6: about 50% of maximal activity Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.2 8.4 stable Homo sapiens

Cofactor

Cofactor Comment Organism Structure
biotin biotin-containing enzyme Homo sapiens
biotin 4 mol of biotin bound per mol of enzyme Homo sapiens