Literature summary for 6.4.1.2 extracted from

Hwang, I.W.; Makishima, Y.; Kato, T.; Park, S.; Terzic, A.; Park, E.Y.
Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation (2014), Appl. Microbiol. Biotechnol., 98, 8201-8209 .

Search for more literature for 6.4.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
citrate	allosteric activation, presence of citrate leads to dose-dependent formation of dimers and tetramers	Homo sapiens

Application

Application	Comment	Organism
synthesis	expression in Bombyx mori using the silkworm NPV bacmid system. Recombinant protein shows biotinylation capacity,and exhibits posttranslational biotinylation and phosphorylation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Bombyx mori	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of the N-terminal 148 hydrophobic amino acids to enhance the solubility of heterologous protein expressed in Bomby mori. Expression of the deletion mutant leads to a high yield of recombinant protein	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O00763	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation decreases ACC2 catalytic activity whereas dephosphorylation activates the enzymatic function by 2fold. Recombinant protein expressed in Bombyx mori exhibits posttranslational phosphorylation	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.786	-	recombinant N-terminal deletion mutant, pH 7.4, 37°C	Homo sapiens
1.336	-	recombinant N-terminal deletion mutant treated with Lambda protein phosphatase, pH 7.4, 37°C	Homo sapiens
1.363	-	recombinant N-terminal deletion mutant, pH 7.4, 37°C, presence of 5 mM citrate	Homo sapiens
2.246	-	recombinant N-terminal deletion mutant, pH 7.4, 37°C, presence of 10 mM citrate	Homo sapiens
4.186	-	recombinant N-terminal deletion mutant, pH 7.4, 37°C, presence of 20 mM citrate	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetyl-CoA + HCO3-	-	Homo sapiens	ADP + phosphate + malonyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
ACACB	-	Homo sapiens
ACC2	-	Homo sapiens

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Release 2023.1 (January 2023)