Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.4.1.2 extracted from

  • Kim, K.W.; Yamane, H.; Zondlo, J.; Busby, J.; Wang, M.
    Expression, purification, and characterization of human acetyl-CoA carboxylase 2 (2007), Protein Expr. Purif., 53, 16-23.
    View publication on PubMed
Search for more literature for 6.4.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Trichoplusia ni cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,3,14,14-tetramethylhexadecanedioic acid
-
 Homo sapiens
malonyl-CoA
-
 Homo sapiens
tall oil fatty acid
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 acetyl-CoA in 50 mM HEPES (pH 7.5), 2.0 mM MgCl2, 2.0 mM ATP, 2.0 mM potassium citrate, 12.5 mM sodium bicarbonate, 1 mg/ml bovine serum albumin, and 0.005 mM acetyl-CoA, at 25°C Homo sapiens
0.052
-
 ATP in 50 mM HEPES (pH 7.5), 2.0 mM MgCl2, 2.0 mM ATP, 2.0 mM potassium citrate, 12.5 mM sodium bicarbonate, 1 mg/ml bovine serum albumin, and 0.005 mM acetyl-CoA, at 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
 SDS-PAGE Homo sapiens

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 20 and 30% glycerol can stabilize the enzyme for a period up to 4 weeks Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O00763
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity resin column chromatography, Q Sepharose HP column chromatography, and Superdex 200 gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00096
-
 cell lysate Homo sapiens
0.00921
-
 after purification Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, in 20 mM Tris-HCl (pH 7.5) containing 0.3M NaCl, 10% glycerol, and 5 mM DTT, 4 days, rapid loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + malonyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
ACC2
-
 Homo sapiens
acetyl-CoA carboxylase 2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.155
-
 ATP in 50 mM HEPES (pH 7.5), 2.0 mM MgCl2, 2.0 mM ATP, 2.0 mM potassium citrate, 12.5 mM sodium bicarbonate, 1 mg/ml bovine serum albumin, and 0.005 mM acetyl-CoA, at 25°C Homo sapiens
0.192
-
 acetyl-CoA in 50 mM HEPES (pH 7.5), 2.0 mM MgCl2, 2.0 mM ATP, 2.0 mM potassium citrate, 12.5 mM sodium bicarbonate, 1 mg/ml bovine serum albumin, and 0.005 mM acetyl-CoA, at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.008
-
-
 Homo sapiens tall oil fatty acid
0.017
-
-
 Homo sapiens malonyl-CoA
0.558
-
-
 Homo sapiens 3,3,14,14-tetramethylhexadecanedioic acid