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Literature summary for 6.4.1.2 extracted from
- Acetyl-coenzyme A carboxylase from the developing endosperm of Ricinus communis. I. Isolation and characterization (1983), Arch. Biochem. Biophys., 225, 576-585.
General Stability
| General Stability | Organism |
|---|---|
| high concentrations of enzyme are required for stability as well as the presence of DTT, glycerol, HCO3-, Triton X-100, and polyvinylpyrrolidone | Ricinus communis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Ricinus communis |  |
| glyoxylate | - |
Ricinus communis | |
| NADH | - |
Ricinus communis | |
| NADPH | - |
Ricinus communis | |
| oxaloacetate | - |
Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
acetyl-CoA | - |
Ricinus communis | |
| 0.1 | - |
ATP | - |
Ricinus communis | |
| 3 | - |
HCO3- | - |
Ricinus communis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Ricinus communis | - |
- |
| plastid membrane | - |
Ricinus communis | 42170 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cs+ | small activating effect, depends on the degree of saturation of the reaction by HCO3- | Ricinus communis | |
| K+ | small activating effect, depends on the degree of saturation of the reaction by HCO3- | Ricinus communis | |
| Mg2+ | both MgATP2- and free Mg2+ required. Maximal activation at 5 mM Mg2+ | Ricinus communis | |
| Mg2+ | Mg2+ or Mn2+ essential for activity | Ricinus communis | |
| Mn2+ | Mn2+ or Mg2+ essential for activity. Maximal activation at 1.5 mM Mn2+ | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 528000 | - |
glycerol density gradient centrifugation | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | endosperm | Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.052 | - |
- |
Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Ricinus communis | ADP + phosphate + malonyl-CoA | - |
? | |
| UTP + acetyl-CoA + HCO3- | at 2.5% of the activity relative to ATP | Ricinus communis | UDP + phosphate + malonyl-CoA | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 28 | transition point | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ricinus communis |
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