General Stability | Organism |
---|---|
DTT, glycerol, Triton X-100, and high concentrations of the enzyme are required for stability | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Glycine max | |
AMP | - |
Glycine max | |
citrate | 10 mM | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | 9686 enzyme | Glycine max | |
0.17 | - |
acetyl-CoA | 9686 enzyme | Glycine max | |
0.32 | - |
acetyl-CoA | Wayne enzyme | Glycine max | |
0.46 | - |
ATP | Wayne enzyme | Glycine max | |
1.13 | - |
HCO3- | Wayne enzyme | Glycine max | |
1.56 | - |
HCO3- | 9686 enzyme | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, 9686 enzyme, SDS-PAGE | Glycine max |
58000 | - |
x * 58000 + x * 65000 + x * 240000, SDS-PAGE | Glycine max |
65000 | - |
x * 58000 + x * 65000 + x * 240000, SDS-PAGE | Glycine max |
240000 | - |
x * 58000 + x * 65000 + x * 240000, SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
2 lines of seeds: 9686 and Wayne | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | 2 lines of seeds: 9686 and Wayne | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | absolute specificity for acetyl-CoA | Glycine max | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + butyryl-CoA + HCO3- | no activity | Glycine max | ADP + ethylmalonyl-CoA + phosphate | - |
? | |
ATP + propionyl-CoA + HCO3- | no activity | Glycine max | ADP + phosphate + methylmalonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000 + x * 65000 + x * 240000, SDS-PAGE | Glycine max |
? | x * 40000, 9686 enzyme, SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
9686 enzyme | Glycine max |
8.2 | - |
Wayne enzyme | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | covalent attachment of the biotin prosthetic group | Glycine max |