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Literature summary for 6.4.1.1 extracted from

  • Gupta, S.; Sharma, A.; Kushwaha, H.; Shukla, P.
    Over-expression of a Codon optimized yeast cytosolic pyruvate carboxylase (PYC2) in CHO cells for an augmented lactate metabolism (2017), Front. Pharmacol., 8, 463 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis super-transfection of CHO cells with a mammalian construct bearing codon optimized yeast cytosolic pyruvate carboxylase PYC2 and a strong fusion promoter for optimal expression of PYC2 enzyme in order to control the lactate metabolism of mAb (IgG1-kappa) producing CHO clones. Presence of Pyc2 results in an improved mAb titer up to 5%, galactosylation up to 2.5folds, and mannosylation up to twofold Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized expression in CHO cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Synonyms

Synonyms Comment Organism
PYC2
-
 Saccharomyces cerevisiae