Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | acetyl-CoA acts to decrease the activation free energy of the reaction by both increasing the activation entropy and decreasing the activation enthalpy | Rhizobium etli |
Protein Variants | Comment | Organism |
---|---|---|
D420A | mutation affects acetyl-CoA binding, enhances the activity of the enzyme in the absence of acetyl-CoA | Rhizobium etli |
R424 | mutation affects acetyl-CoA binding, enhances the activity of the enzyme in the absence of acetyl-CoA. Mutation decreases the activation enthalpy of the pyruvate carboxylation reaction by an amount consistent with removal of a single hydrogen bond | Rhizobium etli |
R429K | enhances the activity of the enzyme in the absence of acetyl-CoA | Rhizobium etli |
R429S | residue Arg429 is especially important for acetyl CoA binding. Mutation results in a 100fold increase in the Ka of acetyl-CoA activation and a large decrease in the cooperativity of this activation | Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | Q2K340 | - |
- |
Rhizobium etli ATCC 51251 | Q2K340 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Rhizobium etli | ADP + oxaloacetate + phosphate | - |
? | |
ATP + pyruvate + HCO3- | - |
Rhizobium etli ATCC 51251 | ADP + oxaloacetate + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
pyruvate | wild-type, pH 7.8, 30°C | Rhizobium etli | |
0.37 | - |
pyruvate | mutant D420A, pH 7.8, 30°C | Rhizobium etli | |
0.51 | - |
pyruvate | mutant R424S, pH 7.8, 30°C | Rhizobium etli | |
0.72 | - |
pyruvate | mutant R429S, pH 7.8, 30°C | Rhizobium etli | |
0.84 | - |
pyruvate | mutant R429K, pH 7.8, 30°C | Rhizobium etli | |
5.67 | - |
pyruvate | mutant R429K, pH 7.8, 30°C, presence of acetyl-CoA | Rhizobium etli | |
5.73 | - |
pyruvate | mutant D420A, pH 7.8, 30°C, presence of acetyl-CoA | Rhizobium etli | |
6.19 | - |
pyruvate | mutant R429S, pH 7.8, 30°C, presence of acetyl-CoA | Rhizobium etli | |
9.8 | - |
pyruvate | mutant R424S, pH 7.8, 30°C, presence of acetyl-CoA | Rhizobium etli | |
13.3 | - |
pyruvate | wild-type, pH 7.8, 30°C, presence of acetyl-CoA | Rhizobium etli |