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Literature summary for 6.4.1.1 extracted from

  • Sirithanakorn, C.; Jitrapakdee, S.; Attwood, P.V.
    Investigation of the roles of allosteric domain arginine, aspartate, and glutamate residues of Rhizobium etli pyruvate carboxylase in relation to its activation by acetyl CoA (2016), Biochemistry, 55, 4220-4228 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA acetyl-CoA acts to decrease the activation free energy of the reaction by both increasing the activation entropy and decreasing the activation enthalpy Rhizobium etli

Protein Variants

Protein Variants Comment Organism
D420A mutation affects acetyl-CoA binding, enhances the activity of the enzyme in the absence of acetyl-CoA Rhizobium etli
R424 mutation affects acetyl-CoA binding, enhances the activity of the enzyme in the absence of acetyl-CoA. Mutation decreases the activation enthalpy of the pyruvate carboxylation reaction by an amount consistent with removal of a single hydrogen bond Rhizobium etli
R429K enhances the activity of the enzyme in the absence of acetyl-CoA Rhizobium etli
R429S residue Arg429 is especially important for acetyl CoA binding. Mutation results in a 100fold increase in the Ka of acetyl-CoA activation and a large decrease in the cooperativity of this activation Rhizobium etli

Organism

Organism UniProt Comment Textmining
Rhizobium etli Q2K340
-
-
Rhizobium etli ATCC 51251 Q2K340
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
Rhizobium etli ADP + oxaloacetate + phosphate
-
?
ATP + pyruvate + HCO3-
-
Rhizobium etli ATCC 51251 ADP + oxaloacetate + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
pyruvate wild-type, pH 7.8, 30°C Rhizobium etli
0.37
-
pyruvate mutant D420A, pH 7.8, 30°C Rhizobium etli
0.51
-
pyruvate mutant R424S, pH 7.8, 30°C Rhizobium etli
0.72
-
pyruvate mutant R429S, pH 7.8, 30°C Rhizobium etli
0.84
-
pyruvate mutant R429K, pH 7.8, 30°C Rhizobium etli
5.67
-
pyruvate mutant R429K, pH 7.8, 30°C, presence of acetyl-CoA Rhizobium etli
5.73
-
pyruvate mutant D420A, pH 7.8, 30°C, presence of acetyl-CoA Rhizobium etli
6.19
-
pyruvate mutant R429S, pH 7.8, 30°C, presence of acetyl-CoA Rhizobium etli
9.8
-
pyruvate mutant R424S, pH 7.8, 30°C, presence of acetyl-CoA Rhizobium etli
13.3
-
pyruvate wild-type, pH 7.8, 30°C, presence of acetyl-CoA Rhizobium etli