Literature summary for 6.4.1.1 extracted from

Tsuji, Y.; Suzuki, I.; Shiraiwa, Y.
Enzymological evidence for the function of a plastid-located pyruvate carboxylase in the Haptophyte alga Emiliania huxleyi: a novel pathway for the production of C4 compounds (2012), Plant Cell Physiol., 53, 1043-1052.

Search for more literature for 6.4.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli and Streptomyces lividans as a His-tagged fusion protein. In Streptomyces lividans a functional His-EhPYC1 is expressed	Emiliania huxleyi

Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxoglutarate	weak inhibition	Emiliania huxleyi
Avidin	-	Emiliania huxleyi
L-glutamate	weak inhibition	Emiliania huxleyi
L-malate	-	Emiliania huxleyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.048	-	ATP	pH 8, 30°C	Emiliania huxleyi
0.47	-	HCO3-	pH 8, 30°C	Emiliania huxleyi
0.88	-	pyruvate	pH 8, 30°C	Emiliania huxleyi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Emiliania huxleyi	9536	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
135000	-	Western blot	Emiliania huxleyi

Organism

Organism	UniProt	Comment	Textmining
Emiliania huxleyi	B9X0T8	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Emiliania huxleyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pyruvate + HCO3-	-	Emiliania huxleyi	ADP + phosphate + oxaloacetate	-	?

Synonyms

Synonyms	Comment	Organism
EhPYC1	-	Emiliania huxleyi

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
6	-	pH 8, 30°C	Emiliania huxleyi	L-malate

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Release 2023.1 (January 2023)