Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Methanocaldococcus jannaschii | |
ATP | - |
Methanocaldococcus jannaschii | |
Avidin | 100 molar excess amount completely inhibits after 5 min | Methanocaldococcus jannaschii | |
KCl | activates at lower concentrations, inhibits at higher concentrations | Methanocaldococcus jannaschii | |
Mg2+ | when Mg2+ concentration in the assay exceeds that of ATP | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
HCO3- | 80°C, pH 8.5 | Methanocaldococcus jannaschii | |
0.374 | - |
ATP | 80°C, pH 8.5 | Methanocaldococcus jannaschii | |
0.53 | - |
pyruvate | 80°C, pH 8.5 | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can poorly replace Mg2+ | Methanocaldococcus jannaschii | |
KCl | optimal concentration: 0.175 mM, activates at lower concentrations, inhibits at higher concentrations | Methanocaldococcus jannaschii | |
Mg2+ | required, inhibits at higher concentrations | Methanocaldococcus jannaschii | |
Mn2+ | can poorly replace Mg2+ | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE | Methanocaldococcus jannaschii |
65000 | - |
alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE | Methanocaldococcus jannaschii |
480000 | - |
gel filtration, Stokes' radius | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | Methanocaldococcus jannaschii | - |
ADP + phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
-20°C, stable, purified enzyme, 75% activity after 5 days | Methanocaldococcus jannaschii |
-80°C, stable, purified enzyme, 75% activity after 5 days | Methanocaldococcus jannaschii |
4°C, stable, purified enzyme, 83-85% activity after 36 h | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Methanocaldococcus jannaschii | ADP + phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 90 | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
rapid inactivation, t1/2: 1 h in 100 mM Tris/HCl, pH 7, sulfate salts provide partial protection | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | - |
Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
ADP | 80°C, pH 8.5 | Methanocaldococcus jannaschii | |
5.34 | - |
ATP | 80°C, pH 8.5 | Methanocaldococcus jannaschii |