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Literature summary for 6.4.1.1 extracted from

  • Mukhopadhyay, B.; Patel, V.J.; Wolfe, R.S.
    A stable archaeal pyruvate carboxylase from the hyperthermophile Methanococcus jannaschii (2000), Arch. Microbiol., 174, 406-414.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Methanocaldococcus jannaschii
ATP
-
Methanocaldococcus jannaschii
Avidin 100 molar excess amount completely inhibits after 5 min Methanocaldococcus jannaschii
KCl activates at lower concentrations, inhibits at higher concentrations Methanocaldococcus jannaschii
Mg2+ when Mg2+ concentration in the assay exceeds that of ATP Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
HCO3- 80°C, pH 8.5 Methanocaldococcus jannaschii
0.374
-
ATP 80°C, pH 8.5 Methanocaldococcus jannaschii
0.53
-
pyruvate 80°C, pH 8.5 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can poorly replace Mg2+ Methanocaldococcus jannaschii
KCl optimal concentration: 0.175 mM, activates at lower concentrations, inhibits at higher concentrations Methanocaldococcus jannaschii
Mg2+ required, inhibits at higher concentrations Methanocaldococcus jannaschii
Mn2+ can poorly replace Mg2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE Methanocaldococcus jannaschii
65000
-
alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE Methanocaldococcus jannaschii
480000
-
gel filtration, Stokes' radius Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + HCO3- Methanocaldococcus jannaschii
-
ADP + phosphate + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Storage Stability

Storage Stability Organism
-20°C, stable, purified enzyme, 75% activity after 5 days Methanocaldococcus jannaschii
-80°C, stable, purified enzyme, 75% activity after 5 days Methanocaldococcus jannaschii
4°C, stable, purified enzyme, 83-85% activity after 36 h Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
Methanocaldococcus jannaschii ADP + phosphate + oxaloacetate
-
?

Subunits

Subunits Comment Organism
octamer alpha4,beta4, 4 * 55000 + 4 * 65000, the 65000 Da subunit is biotinylated, the 55000 Da subunit is not, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
rapid inactivation, t1/2: 1 h in 100 mM Tris/HCl, pH 7, sulfate salts provide partial protection Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
biotin
-
Methanocaldococcus jannaschii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.89
-
ADP 80°C, pH 8.5 Methanocaldococcus jannaschii
5.34
-
ATP 80°C, pH 8.5 Methanocaldococcus jannaschii