Cloned (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
General Stability | Organism |
---|---|
acetyl-CoA protects against thermal denaturation | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Rhodobacter capsulatus | |
ADP | - |
Rhodobacter capsulatus | |
Avidin | - |
Rhodobacter capsulatus | |
L-Asp | - |
Rhodobacter capsulatus | |
Mn2+ | inhibition above 1.25 mM, activation below | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | completely dependent on divalent metal ion, Mg2+ or Mn2+ | Rhodobacter capsulatus | |
Mn2+ | divalent metal ion required, Mg2+ or Mn2+. Mn2+ inhibits at concentrations above 1.25 mM | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
overexpressing strain | - |
Purification (Comment) | Organism |
---|---|
rapid dye-ligand affinity chromatography procedure | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Rhodobacter capsulatus | ADP + phosphate + oxaloacetate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
acetyl-CoA protects against thermal inactivation | Rhodobacter capsulatus |
20 | - |
1 h, 75% inactivation | Rhodobacter capsulatus |
50 | - |
15 min, complete loss of activity | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activated by MnCl2 | Rhodobacter capsulatus |
8 | - |
activated by Mg2+, at a MgCl2 concentration double that of ATP | Rhodobacter capsulatus |
8.5 | - |
activated by Mg2+ equimolar to ATP | Rhodobacter capsulatus |