BRENDA - Enzyme Database
show all sequences of 6.3.5.9

Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase

Crouzet, J.; Cauchois, L.; Blanche, F.; Debussche, L.; Thibaut, D.; Rouyez, M.C.; Rigault, S.; Mayaux, J.F.; Cameron, B.; J. Bacteriol. 172, 5968-5979 (1990)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
identification of the structural gene
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
additional information
Pseudomonas denitrificans (nomen rejiciendum) SC510
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
P21632
-
-
Pseudomonas denitrificans (nomen rejiciendum) SC510
P21632
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Cloned(Commentary) (protein specific)
Commentary
Organism
identification of the structural gene
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
additional information
Pseudomonas denitrificans (nomen rejiciendum) SC510
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Other publictions for EC 6.3.5.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
661083
Fresquet
Mechanism of cobyrinic acid a, ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochemistry
43
10619-10627
2004
-
-
1
-
6
-
-
8
-
-
2
-
-
42
-
-
-
-
-
-
-
-
8
1
-
-
-
8
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
8
-
-
2
-
-
-
-
-
-
-
-
-
8
1
-
-
-
8
1
-
-
-
-
-
-
-
-
-
653832
Galperin
The synthetase domains of coba ...
Escherichia coli
Proteins
41
238-247
2000
-
-
-
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
651906
Tsang
CobB, a new member of the SIR2 ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
273
31788-31794
1998
-
-
1
-
-
-
-
-
-
-
2
-
-
42
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651627
Tsang
cobB function is required for ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Bacteriol.
178
7016-7019
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
42
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651618
Blanche
Biosynthesis of vitamin B12: s ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 RifT
J. Bacteriol.
173
6046-6051
1991
-
-
1
-
-
1
-
13
-
1
2
1
-
2
-
-
1
-
-
-
2
3
7
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
13
-
1
2
1
-
-
-
1
-
-
2
3
7
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
485086
Crouzet
Nucleotide sequence of a Pseud ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510
J. Bacteriol.
172
5968-5979
1990
-
-
1
-
-
-
-
-
-
-
1
2
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644524
Debussche
Purification and characterizat ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
172
6239-6244
1990
-
-
1
-
-
-
-
7
-
1
2
2
-
2
-
-
1
-
-
-
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
1
2
2
-
-
-
1
-
-
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-