BRENDA - Enzyme Database
show all sequences of 6.3.5.7

Plasmodium apicoplast Gln-tRNAGln biosynthesis utilizes a unique GatAB amidotransferase essential for erythrocytic stage parasites

Mailu, B.M.; Li, L.; Arthur, J.; Nelson, T.M.; Ramasamy, G.; Fritz-Wolf, K.; Becker, K.; Gardner, M.J.; J. Biol. Chem. 290, 29629-29641 (2015)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium berghei
20011
-
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium falciparum
20011
-
Organism
Organism
UniProt
Commentary
Textmining
Plasmodium berghei
A0A509AHQ9 and A0A509AM58
A0A077X9J8 i.e. subunit GatA, A0A077XDD6 i.e. subunit GatB
-
Plasmodium berghei ANKA
A0A509AHQ9 and A0A509AM58
A0A077X9J8 i.e. subunit GatA, A0A077XDD6 i.e. subunit GatB
-
Plasmodium falciparum
Q8I1S6 and C6KTC3
Q8I1S6 i.e. subunit GatA, C6KTC3 i.e. subunit GatB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium falciparum
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei ANKA
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
additional information
no substrate: L-glutamate
745335
Plasmodium falciparum
?
-
-
-
-
additional information
no substrate: L-glutamate
745335
Plasmodium berghei
?
-
-
-
-
additional information
no substrate: L-glutamate
745335
Plasmodium berghei ANKA
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
1 * 85000, subunit GatA, 1 * 94500, subunit GatB, SDS-PAGE
Plasmodium falciparum
Synonyms
Synonyms
Commentary
Organism
BANKA_112750
subunit GatB, gene name
Plasmodium berghei
PBANKA_071810
subunit GatA, gene name
Plasmodium berghei
PF3D7_0416100
subunit GatA, gene name
Plasmodium falciparum
PF3D7_0628800
subunit GatB, gene name
Plasmodium falciparum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium berghei
20011
-
apicoplast
in blood stage parasites, heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits
Plasmodium falciparum
20011
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium falciparum
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
ATP + L-glutamyl-tRNAGln + L-glutamine
-
745335
Plasmodium berghei ANKA
ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
-
-
?
additional information
no substrate: L-glutamate
745335
Plasmodium falciparum
?
-
-
-
-
additional information
no substrate: L-glutamate
745335
Plasmodium berghei
?
-
-
-
-
additional information
no substrate: L-glutamate
745335
Plasmodium berghei ANKA
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 85000, subunit GatA, 1 * 94500, subunit GatB, SDS-PAGE
Plasmodium falciparum
General Information
General Information
Commentary
Organism
physiological function
gene encoding GatA is essential to the Plasmodium berghei blood stages
Plasmodium berghei
General Information (protein specific)
General Information
Commentary
Organism
physiological function
gene encoding GatA is essential to the Plasmodium berghei blood stages
Plasmodium berghei
Other publictions for EC 6.3.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745335
Mailu
Plasmodium apicoplast Gln-tRN ...
Plasmodium berghei, Plasmodium berghei ANKA, Plasmodium falciparum
J. Biol. Chem.
290
29629-29641
2015
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1
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1
1
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745612
Dewage
Computational analysis of amm ...
Staphylococcus aureus, Staphylococcus aureus ATCC 700699
J. Phys. Chem. B
119
3669-3677
2015
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1
4
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743955
Chongdar
Preliminary X-ray crystallogr ...
Escherichia coli
Acta Crystallogr. Sect. F
70
922-927
2014
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1
1
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744268
Echevarria
Glutamyl-tRNAGln amidotransfe ...
Mus musculus
Biochem. J.
460
91-101
2014
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4
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1
1
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745552
Hadd
Coevolution of specificity de ...
Saccharomyces cerevisiae
J. Mol. Biol.
426
3619-3633
2014
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1
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4
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1
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2
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2
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1
1
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2
2
728154
Grant
The structure of yeast glutami ...
Thermotoga maritima
J. Mol. Biol.
425
2480-2493
2013
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728391
Huot
Gln-tRNAGln synthesis in a dyn ...
Helicobacter pylori
Nucleic Acids Res.
39
9306-9315
2011
1
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3
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1
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2
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728668
O'Donoghue
Rational design of an evolutio ...
Methanothermobacter thermautotrophicus
Proc. Natl. Acad. Sci. USA
108
20485-20490
2011
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1
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1
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4
4
709508
Chatani
A simple turbidimetric method ...
Staphylococcus aureus
J. Microbiol. Methods
80
117-122
2010
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714451
Balg
Inhibition of Helicobacter pyl ...
Helicobacter pylori
Bioorg. Med. Chem.
18
7868-7872
2010
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7
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7
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716331
Ito
Two enzymes bound to one trans ...
Thermotoga maritima
Nature
467
612-616
2010
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1
1
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716371
Rampias
The archaeal transamidosome fo ...
Methanothermobacter thermautotrophicus
Nucleic Acids Res.
38
5774-5783
2010
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1
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2
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3
3
706514
Nagao
Biogenesis of glutaminyl-mt tR ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
16209-16214
2009
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3
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3
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692759
Balg
Inhibition of Helicobacter pyl ...
Helicobacter pylori
J. Am. Chem. Soc.
130
3264-3265
2008
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8
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673689
Namgoong
Co-evolution of the archaeal t ...
Methanothermobacter thermautotrophicus
FEBS Lett.
581
309-314
2007
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674787
Sheppard
The Helicobacter pylori amidot ...
Helicobacter pylori
J. Biol. Chem.
282
11866-11873
2007
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662450
Feng
Gln-tRNAGln formation from Glu ...
Methanothermobacter thermautotrophicus
J. Biol. Chem.
280
8150-8155
2005
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492304
Harpel
Mutagenesis and mechanism-base ...
Streptococcus pyogenes
Biochemistry
41
6398-6407
2002
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492305
Kwak
Expression, purification, and ...
Geobacillus stearothermophilus
Mol. Cells
14
374-381
2002
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1
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492302
Horiuchi
Mechanistic studies of reactio ...
Streptococcus pyogenes
Biochemistry
40
6450-6457
2001
1
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5
3
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1
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492308
Salazar
A dual-specific Glu-tRNA(Gln) ...
Acidithiobacillus ferrooxidans
FEBS Lett.
500
129-131
2001
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1
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492303
Curnow
Glu-tRNAGln amidotransferase: ...
Bacillus subtilis
Proc. Natl. Acad. Sci. USA
94
11819-11826
1997
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