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Literature summary for 6.3.5.7 extracted from

  • Huot, J.L.; Fischer, F.; Corbeil, J.; Madore, E.; Lorber, B.; Diss, G.; Hendrickson, T.L.; Kern, D.; Lapointe, J.
    Gln-tRNAGln synthesis in a dynamic transamidosome from Helicobacter pylori, where GluRS2 hydrolyzes excess Glu-tRNAGln (2011), Nucleic Acids Res., 39, 9306-9315.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.7

Activating Compound

Activating Compound Comment Organism Structure
additional information the presence of 0.0066 mM GluRS2 increases the KM of the enzyme for Glu-tRNAGln nearly 4fold Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
 Glu-tRNAGln in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori
0.00097
-
 Glu-tRNAGln in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori
0.00134
-
 Glu-tRNAGln in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Glu-tRNAGln + L-glutamine + H2O Helicobacter pylori
-
 ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, Ni-NTA resin column chromatography, and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Glu-tRNAGln + L-glutamine + H2O
-
 Helicobacter pylori ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
GatCAB
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
 Glu-tRNAGln in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori
0.21
-
 Glu-tRNAGln in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori
0.23
-
 Glu-tRNAGln in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Helicobacter pylori