BRENDA - Enzyme Database
show all sequences of 6.3.5.6

The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln

Sheppard, K.; Akochy, P.M.; Salazar, J.C.; Soell, D.; J. Biol. Chem. 282, 11866-11873 (2007)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Helicobacter pylori
S152A
mutant is glutaminase inactive
Helicobacter pylori
S152T
mutant is glutaminase inactive
Helicobacter pylori
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00095
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.00118
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.0207
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.0224
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.0402
-
Gln
37C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
0.0509
-
Gln
37C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity
Helicobacter pylori
0.2068
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Helicobacter pylori
-
-
-
Purification (Commentary)
Commentary
Organism
-
Helicobacter pylori
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + Asp-tRNAAsn + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
674787
Helicobacter pylori
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
?
ATP + Glu-tRNAGln + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
674787
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.027
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.052
2.1
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
1.3
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
3
6
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
3.49
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
3.61
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.1
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
10.29
-
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
11.8
-
Gln
37C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Helicobacter pylori
S152A
mutant is glutaminase inactive
Helicobacter pylori
S152T
mutant is glutaminase inactive
Helicobacter pylori
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00095
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.00118
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.0207
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.0224
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.0402
-
Gln
37C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
0.0509
-
Gln
37C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity
Helicobacter pylori
0.2068
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
Purification (Commentary) (protein specific)
Commentary
Organism
-
Helicobacter pylori
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + Asp-tRNAAsn + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
674787
Helicobacter pylori
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
?
ATP + Glu-tRNAGln + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
674787
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.027
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
0.052
2.1
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
1.3
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
3
6
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
3.49
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
3.61
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.1
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
10.29
-
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
11.8
-
Gln
37C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
Other publictions for EC 6.3.5.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746380
Vijayakumar
Soluble expression and purifi ...
Fasciola gigantica
Protein Expr. Purif.
143
9-13
2018
-
-
1
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1
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1
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1
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728743
Fuengfuloy
Overproduction of the N-termin ...
no activity in Helicobacter pylori
Protein Expr. Purif.
89
25-32
2013
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1
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728397
Fischer
The asparagine-transamidosome ...
Helicobacter pylori
Nucleic Acids Res.
40
4965-4976
2012
-
-
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1
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1
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2
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1
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1
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728424
Yu
New WS9326A congeners from Str ...
Brugia malayi
Org. Lett.
14
4946-4949
2012
-
-
-
-
-
-
4
-
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1
-
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4
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715924
Crepin
A hybrid structural model of t ...
Brugia malayi
J. Mol. Biol.
405
1056-1069
2011
-
-
1
1
1
-
-
-
-
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1
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1
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1
1
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716420
Yu
Tirandamycins from Streptomyce ...
Brugia malayi
Org. Lett.
13
2034-2037
2011
-
1
-
-
-
-
1
-
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1
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1
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1
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1
1
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716885
Polydorides
Computational protein design w ...
Thermus thermophilus
Proteins
79
3448-3468
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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-
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-
-
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-
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-
673689
Namgoong
Co-evolution of the archaeal t ...
Methanothermobacter thermautotrophicus
FEBS Lett.
581
309-314
2007
-
-
-
-
-
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3
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1
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1
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674787
Sheppard
The Helicobacter pylori amidot ...
Helicobacter pylori
J. Biol. Chem.
282
11866-11873
2007
-
-
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3
-
-
7
-
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3
-
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1
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2
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9
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3
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7
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1
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2
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9
-
-
-
-
-
-
-
-
-
-
675112
Bernard
Inhibition by L-aspartol adeny ...
Pseudomonas aeruginosa
J. Enzyme Inhib. Med. Chem.
22
77-82
2007
-
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1
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3
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2
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675931
Bailly
The transamidosome: a dynamic ...
Thermus thermophilus
Mol. Cell
28
228-239
2007
-
-
-
-
-
-
-
1
-
1
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1
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2
1
1
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1
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1
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1
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1
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1
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2
1
1
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1
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672190
Chuawong
The nondiscriminating aspartyl ...
Helicobacter pylori
Biochemistry
45
8079-8087
2006
-
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1
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1
-
1
-
5
-
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3
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1
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1
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1
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3
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675227
Ramirez
Brugia malayi asparaginyl-tran ...
Brugia malayi, Homo sapiens
J. Infect. Dis.
193
1164-1171
2006
-
-
2
-
2
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1
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2
-
2
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3
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2
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2
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2
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1
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3
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2
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677106
Cardoso
A non-discriminating aspartyl- ...
Halobacterium salinarum, Halobacterium salinarum NRC 1
RNA Biol.
3
110-114
2006
-
-
-
-
-
-
-
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1
-
2
-
4
-
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6
-
1
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1
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1
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1
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1
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2
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6
-
1
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1
-
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-
-
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-
659057
Akochy
Direct glutaminyl-tRNA biosynt ...
Pseudomonas aeruginosa
J. Bacteriol.
186
767-776
2004
-
-
1
-
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1
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1
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1
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2
1
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-
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440091
Tumbula-Hansen
Evolutionary divergence of the ...
Methanothermobacter thermautotrophicus
J. Biol. Chem.
277
37184-37190
2002
-
-
1
-
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2
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1
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1
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3
1
1
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1
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2
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1
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3
1
1
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-
-
-
-
-
-
-
-
-
-
-
440093
Min
Transfer RNA-dependent amino a ...
Deinococcus radiodurans, Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539, Thermus thermophilus
Proc. Natl. Acad. Sci. USA
99
2678-2683
2002
-
-
1
-
-
-
-
-
-
-
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6
-
9
-
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-
-
-
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9
2
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1
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6
-
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9
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
440092
Raczniak
A single amidotransferase form ...
Chlamydia trachomatis
J. Biol. Chem.
276
45862-45867
2001
-
1
1
-
-
-
1
-
-
1
2
4
-
1
-
-
1
-
-
-
1
-
8
1
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
1
2
4
-
-
-
1
-
-
1
-
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
440090
Becker
The heterotrimeric Thermus the ...
Bacillus subtilis, Deinococcus radiodurans, Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
FEBS Lett.
476
140-144
2000
-
-
1
-
1
-
-
-
-
-
-
6
-
96
-
-
-
-
-
-
-
-
17
4
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
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6
-
-
-
-
-
-
-
-
17
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
440094
Ibba
Aminoacyl-tRNA synthesis ...
Aeropyrum pernix, Archaeoglobus fulgidus, Bacillus subtilis, Deinococcus radiodurans, Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, Thermus thermophilus
Annu. Rev. Biochem.
69
617-650
2000
-
-
-
-
-
-
-
-
-
-
-
3
-
7
-
-
-
-
-
-
-
-
20
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
20
7
-
-
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-
440095
Tumbula
Domain-specific recruitment of ...
Aeropyrum pernix, Archaeoglobus fulgidus, Bacillus subtilis, Chlamydia trachomatis, Deinococcus radiodurans, Halobacterium salinarum, Helicobacter pylori, Methanocaldococcus jannaschii, Methanosarcina mazei, Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus DELTAH, Saccharolobus solfataricus, Sulfurisphaera tokodaii
Nature
407
106-110
2000
-
-
10
-
-
-
-
-
-
-
-
8
-
25
-
-
1
-
-
-
-
-
35
7
2
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
35
7
2
-
-
-
-
-
-
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-
-
-
440087
Curnow
Glutamyl-tRNAGln amidotransfer ...
Bacillus subtilis, Deinococcus radiodurans, Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539, Haloferax volcanii
Proc. Natl. Acad. Sci. USA
95
12838-12843
1998
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1
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3
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13
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1
-
-
-
-
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15
-
1
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
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-
15
-
1
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-