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Literature summary for 6.3.5.6 extracted from

  • Chuawong, P.; Hendrickson, T.L.
    The nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori: anticodon-binding domain mutations that impact tRNA specificity and heterologous toxicity (2006), Biochemistry, 45, 8079-8087.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3), co-expression with the cytotoxic nondiscriminating aspartyl-tRNA synthetase, EC 6.1.1.23, which is toxic for the cells, rescues the toxicity, overview Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartyl-tRNAAsn + L-glutamine Helicobacter pylori
-
 ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartyl-tRNAAsn + L-glutamine
-
 Helicobacter pylori ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
 ?
ATP + L-aspartyl-tRNAAsn + L-glutamine the Halobacter pylori Asp/Glu-Adt can utilize Escherichia coli Asp-tRNAAsn as a substrate, recombinantly produced tRNA substrate Helicobacter pylori ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
 ?
additional information in bacteria that lack AsnRS, EC 6.1.1.12, AspRS is nondiscriminating, EC 6.1.1.23, and generates both Asp-tRNAAsp and the noncanonical, misacylated Asp-tRNAAsn, this misacylated tRNA is subsequently repaired by the glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, overview Helicobacter pylori ?
-
 ?

Synonyms

Synonyms Comment Organism
Asp/Glu-Adt
-
 Helicobacter pylori
glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase
-
 Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Helicobacter pylori