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Literature summary for 6.3.5.6 extracted from
- Domain-specific recruitment of amide amino acids for protein synthesis (2000), Nature, 407, 106-110.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of the gatCAB genes encoding the Asp/Glu-AdT and overexpression in Escherichia coli | Methanothermobacter thermautotrophicus |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Bacillus subtilis |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Methanothermobacter thermautotrophicus |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Halobacterium salinarum |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Saccharolobus solfataricus |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Methanocaldococcus jannaschii |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Archaeoglobus fulgidus |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Deinococcus radiodurans |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Methanosarcina mazei |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Aeropyrum pernix |
| presence of the gatCAB genes encoding Asp/Glu-AdT | Sulfurisphaera tokodaii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Asp-tRNAAsn + ? | Methanothermobacter thermautotrophicus | - |
Asn-tRNAAsn + ? | - |
? |  |
| Asp-tRNAAsn + ? | Deinococcus radiodurans | tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | Chlamydia trachomatis | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | Helicobacter pylori | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | Deinococcus radiodurans | in vivo only Asn-tRNAAsn formation occurs | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | Methanothermobacter thermautotrophicus DELTAH | - |
Asn-tRNAAsn + ? | - |
? | |
| Glu-tRNAGln + ? | Chlamydia trachomatis | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Gln-tRNAGln + ? | - |
? | |
| Glu-tRNAGln + ? | Helicobacter pylori | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Gln-tRNAGln + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | - |
- |
- |
| Archaeoglobus fulgidus | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Chlamydia trachomatis | - |
- |
- |
| Deinococcus radiodurans | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Helicobacter pylori | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanosarcina mazei | - |
- |
- |
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus | - |
Methanobacterium thermoautotrophicum | - |
| Methanothermobacter thermautotrophicus DELTAH | - |
- |
- |
| Saccharolobus solfataricus | - |
- |
- |
| Sulfurisphaera tokodaii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of recombinant enzyme, expressed in Escherichia coli | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Asp-tRNAAsn + ? | - |
Methanothermobacter thermautotrophicus | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA | Deinococcus radiodurans | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Chlamydia trachomatis | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Helicobacter pylori | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | in vivo only Asn-tRNAAsn formation occurs | Deinococcus radiodurans | Asn-tRNAAsn + ? | - |
? | |
| Asp-tRNAAsn + ? | - |
Methanothermobacter thermautotrophicus DELTAH | Asn-tRNAAsn + ? | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Bacillus subtilis | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Halobacterium salinarum | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Saccharolobus solfataricus | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Methanocaldococcus jannaschii | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Archaeoglobus fulgidus | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Methanosarcina mazei | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Aeropyrum pernix | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | - |
Sulfurisphaera tokodaii | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT | Deinococcus radiodurans | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Chlamydia trachomatis | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Methanothermobacter thermautotrophicus | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Helicobacter pylori | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Deinococcus radiodurans | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Methanothermobacter thermautotrophicus DELTAH | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
| ATP + Glu-tRNAGln + glutamine | - |
Deinococcus radiodurans | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
| ATP + Glu-tRNAGln + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Chlamydia trachomatis | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
| ATP + Glu-tRNAGln + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Methanothermobacter thermautotrophicus | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
| ATP + Glu-tRNAGln + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Helicobacter pylori | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
| ATP + Glu-tRNAGln + glutamine | Asp/Glu-AdT carries out Gln-tRNA formation, since Bacillus contains a non-discriminating GluRS, but lacks a mischarging AspRS | Bacillus subtilis | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
| ATP + Glu-tRNAGln + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Methanothermobacter thermautotrophicus DELTAH | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
| Glu-tRNAGln + ? | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Chlamydia trachomatis | Gln-tRNAGln + ? | - |
? | |
| Glu-tRNAGln + ? | it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo | Helicobacter pylori | Gln-tRNAGln + ? | - |
? | |
| additional information | - |
Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | - |
Archaeoglobus fulgidus | ? | - |
? | |
| additional information | - |
Aeropyrum pernix | ? | - |
? | |
| additional information | no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu | Chlamydia trachomatis | ? | - |
? | |
| additional information | no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu | Methanothermobacter thermautotrophicus | ? | - |
? | |
| additional information | no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu | Deinococcus radiodurans | ? | - |
? | |
| additional information | no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu | Methanothermobacter thermautotrophicus DELTAH | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | - |
Chlamydia trachomatis |
| heterotrimer | - |
Methanocaldococcus jannaschii |
| heterotrimer | - |
Archaeoglobus fulgidus |
| heterotrimer | - |
Aeropyrum pernix |
| heterotrimer | GatCAB enzyme | Bacillus subtilis |
| heterotrimer | GatCAB enzyme | Methanothermobacter thermautotrophicus |
| heterotrimer | GatCAB enzyme | Deinococcus radiodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Chlamydia trachomatis |
| 37 | - |
assay at | Methanothermobacter thermautotrophicus |
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