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Literature summary for 6.3.5.6 extracted from

  • Curnow, A.W.; Tumbula, D.L.; Pelaschier, J.T.; Min, B.; Söll, D.
    Glutamyl-tRNAGln amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis (1998), Proc. Natl. Acad. Sci. USA, 95, 12838-12843.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.6

Cloned(Commentary)

Cloned (Comment) Organism
gatCAB genes encoding Glu/Asp-AdT are cloned and expressed in Escherichia coli Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Asp-tRNAAsn + ? Deinococcus radiodurans gatCAB genes encoded Asp-AdT may be responsible for biosynthesis of asparagine, glutamine as amide source Asn-tRNAAsn + ?
-
 ?
Asp-tRNAAsn + ? Deinococcus radiodurans enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Asn-tRNAAsn + ?
-
 ?
Asp-tRNAAsn + ? Deinococcus radiodurans in vivo only Asn-tRNAAsn formation, because the substrate availability of Asp-tRNAAsn allows only one final product Asn-tRNAAsn + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Deinococcus radiodurans
-
-
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
-
-
Haloferax volcanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of recombinant enzyme, expressed in Escherichia coli Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asp-tRNAAsn + ? gatCAB genes encoded Asp-AdT may be responsible for biosynthesis of asparagine, glutamine as amide source Deinococcus radiodurans Asn-tRNAAsn + ?
-
 ?
Asp-tRNAAsn + ? enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Deinococcus radiodurans Asn-tRNAAsn + ?
-
 ?
Asp-tRNAAsn + ? in vivo only Asn-tRNAAsn formation, because the substrate availability of Asp-tRNAAsn allows only one final product Deinococcus radiodurans Asn-tRNAAsn + ?
-
 ?
ATP + Asp-tRNAAsn + glutamine
-
 Bacillus subtilis ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
ATP + Asp-tRNAAsn + glutamine
-
 Haloferax volcanii ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
ATP + Asp-tRNAAsn + glutamine single enzyme with both Gln-tRNA and Asp-tRNA transamidation activities, but functions as Asp-AdT, since Deinococcus do not produce the required mischarged Glu-tRNAGln substrate Deinococcus radiodurans ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Deinococcus radiodurans ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
ATP + Asp-tRNAAsn + glutamine single enzyme with both Gln-tRNA and Asp-tRNA transamidation activities, but functions as Asp-AdT, since Deinococcus do not produce the required mischarged Glu-tRNAGln substrate Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
ATP + Glu-tRNAGln + glutamine dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT Deinococcus radiodurans ADP + Gln-tRNAGln + glutamate + phosphate
-
 ?
ATP + Glu-tRNAGln + glutamine in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Gln-tRNAGln formation Bacillus subtilis ADP + Gln-tRNAGln + glutamate + phosphate
-
 ?
ATP + Glu-tRNAGln + glutamine Asp/Glu-AdT carries out Gln-tRNA formation, since Bacillus contains a non-discriminating GluRS, but lacks a mischarging AspRS Bacillus subtilis ADP + Gln-tRNAGln + glutamate + phosphate
-
 ?
ATP + Glu-tRNAGln + glutamine dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ADP + Gln-tRNAGln + glutamate + phosphate
-
 ?
additional information A subunit is a tRNA-independent glutaminase, B subunit is a putative tRNA-binding protein Deinococcus radiodurans ?
-
 ?
additional information A subunit is a tRNA-independent glutaminase, B subunit is a putative tRNA-binding protein Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Deinococcus radiodurans