Protein Variants | Comment | Organism |
---|---|---|
S44A | mutant of hybrid enzyme shows km value similar to the wild-type hybrid, the kcat values of glutamine and ATP are 14fold lower in comparison to wild-type hybrid, the functional linkage that coordinates the reactions on the glutaminase and carbamoyl-phosphate synthetase domains is lost as a result of mutation | Mammalia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mammalia | |
0.111 | - |
L-glutamine | pH 7.4, 37°C, mutant hybrid enzyme | Mammalia | |
0.114 | - |
L-glutamine | pH 7.4, 37°C, wild-type hybrid enzyme | Mammalia | |
1.66 | - |
ATP | pH 7.4, 37°C, wild-type hybrid enzyme | Mammalia | |
1.83 | - |
ATP | pH 7.4, 37°C, mutant hybrid enzyme | Mammalia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mammalia | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | formation of a hybrid complex consisting of the mammalian glutaminase domain and the isolated Escherichia coli carbamoyl-phosphate synthetase domains, the steady state kinetic parameters of the hybrid are similar to those obtained for carbamoyl-phosphate synthase-aspartate carbamoyltransferase-dihydroorotase | Mammalia |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate | the mammalian enzyme is part of carbamoyl-phosphate synthase-aspartate carbamoyltransferase-dihydroorotase, CAD, the carbamoyl phosphate synthesis requires the concerted action of the glutaminase and carbamoyl-phosphate synthetase domains of CAD | Mammalia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Mammalia | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Mammalia | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mammalia | |
164 | - |
ATP | pH 7.4, 37°C, mutant hybrid enzyme | Mammalia | |
193 | - |
L-glutamine | pH 7.4, 37°C, mutant hybrid enzyme | Mammalia | |
2500 | - |
ATP | pH 7.4, 37°C, wild-type hybrid enzyme | Mammalia | |
2660 | - |
L-glutamine | pH 7.4, 37°C, wild-type hybrid enzyme | Mammalia |