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Literature summary for 6.3.5.5 extracted from

  • Sahay, N.; Guy, H.I.; Liu, X.; Evans, D.R.
    Regulation of an Escherichia coli/mammalian chimeric carbamoyl-phosphate synthetase (1998), J. Biol. Chem., 273, 31195-31202.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphoribosyl 5'-diphosphate activation of Escherichia coli and chimeric enzyme, the presence of UTP reduces the PRPP binding Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information a chimeric deletion mutant A1A2B3 lacks 59 of 163 residues from the carboxyl end of the Escherichia coli /mammalian chimera, this mutant is somewhat more active than the full-length chimeric protein but has reduced sensitivity to allosteric effectors Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
UMP not chimeric protein Escherichia coli
UTP inhibits activity of Escherichia coli and chimeric enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
ATP
-
Escherichia coli
0.46
-
ATP chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors Escherichia coli
0.53
-
ATP deletion mutant, in comparison to the presence of effectors Escherichia coli
2.14
-
ATP
-
Mammalia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, SDS-PAGE, chimeric protein Escherichia coli
120000
-
chimeric protein Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mammalia
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification construction of a chimeric enzyme consisting of Escherichia coli subdomains A1 and A2 fused to the mammalian enzyme subdomain B3 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Mammalia 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + NH4+ + HCO3-
-
Mammalia 2 ADP + phosphate + carbamoyl phosphate
-
?
2 ATP + NH4+ + HCO3-
-
Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE, chimeric protein Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
ATP
-
Mammalia
3.35
-
ATP chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors Escherichia coli
5.3
-
ATP deletion mutant, in comparison to the presence of effectors Escherichia coli
6.08
-
ATP
-
Mammalia
8
-
ATP
-
Escherichia coli