Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphoribosyl 5'-diphosphate | activation of Escherichia coli and chimeric enzyme, the presence of UTP reduces the PRPP binding | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a chimeric deletion mutant A1A2B3 lacks 59 of 163 residues from the carboxyl end of the Escherichia coli /mammalian chimera, this mutant is somewhat more active than the full-length chimeric protein but has reduced sensitivity to allosteric effectors | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | not chimeric protein | Escherichia coli | |
UTP | inhibits activity of Escherichia coli and chimeric enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
ATP | - |
Escherichia coli | |
0.46 | - |
ATP | chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors | Escherichia coli | |
0.53 | - |
ATP | deletion mutant, in comparison to the presence of effectors | Escherichia coli | |
2.14 | - |
ATP | - |
Mammalia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE, chimeric protein | Escherichia coli |
120000 | - |
chimeric protein | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mammalia | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | construction of a chimeric enzyme consisting of Escherichia coli subdomains A1 and A2 fused to the mammalian enzyme subdomain B3 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Mammalia | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Mammalia | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE, chimeric protein | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
ATP | - |
Mammalia | |
3.35 | - |
ATP | chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors | Escherichia coli | |
5.3 | - |
ATP | deletion mutant, in comparison to the presence of effectors | Escherichia coli | |
6.08 | - |
ATP | - |
Mammalia | |
8 | - |
ATP | - |
Escherichia coli |