Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-ornithine | binds to the enzymes large subunit at the interface located between the allosteric and the carbamoyl phosphate domains | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
X-ray crystallographic analysis, the three active sites on the protein are widely separated from one another | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | IMP binds to enzyme but the actual effect of this ligand is dependent upon temperature and assay conditions | Escherichia coli | |
UMP | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | Escherichia coli | the product carbamoylphosphate is utilized in the synthesis of arginine and pyrimidine nucleotides and in the urea cycle | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | the product carbamoylphosphate is utilized in the synthesis of arginine and pyrimidine nucleotides and in the urea cycle | Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alpha,beta, that readily converts to an tetrameric species: 4 * alpha, beta in presence of positive allosteric effectors, the smaller of two subunits contains 382 amino acid residues and is responsible for the hydrolysis of free ammonia, the larger subunit contains 1073 amino acid residues and provides the binding sites for the two MgATP2 molecules required for the assembly of carbamoyl phosphate, structure and reaction mechanism | Escherichia coli |