Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | enhances activity | Escherichia coli | |
ITP | enhances activity | Escherichia coli | |
NEM | 250fold activation of glutaminase activity. Irreversible inactivation of synthetase activity | Escherichia coli | |
Orn | activates | Escherichia coli | |
XMP | enhances activity | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxonorleucine | selective inactivation of Gln-dependent activity | Escherichia coli | |
Alkyl hydrazines | inhibits Gln-dependent activity, but not NH4+-dependent activity | Escherichia coli | |
azaserine | selective inactivation of Gln-dependent activity | Escherichia coli | |
H2O2 | 0.2 mM, inhibits Gln-dependent activity. No effect on the activity with NH4+ in carbamoyl-phosphate synthase reaction | Escherichia coli | |
hydroxylamine | inhibits Gln-dependent activity, but not NH4+-dependent activity | Escherichia coli | |
L-2-Amino-4-oxo-5-chloropentanoate | selective inactivation of Gln-dependent activity | Escherichia coli | |
NEM | irreversible inactivation of synthetase activity. Increase of glutaminase activity | Escherichia coli | |
potassium cyanate | inhibits Gln-dependent activity, but not NH4+-dependent activity | Escherichia coli | |
UDP | inhibits to a lesser extent than UMP | Escherichia coli | |
UMP | phosphate increases inhibition | Escherichia coli | |
UTP | inhibits to a lesser extent than UMP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
L-Gln | - |
Escherichia coli | |
93 | - |
NH4+ | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal concentration: 0.1 M | Escherichia coli | |
NH4+ | activates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000 + 1 * 130000, SDS-PAGE | Escherichia coli |
130000 | - |
1 * 40000 + 1 * 130000, SDS-PAGE | Escherichia coli |
163000 | - |
sedimentation equilibrium ultracentrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
Gln-dependent function of carbamoyl-phosphate synthase is inactivated by incubating the enzyme in air with low concentrations of dithiothreitol, glutathione, Cys, homocysteine, or 2-mercaptoethanol | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | - |
Escherichia coli B / ATCC 11303 | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli B / ATCC 11303 | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 40000 + 1 * 130000, SDS-PAGE | Escherichia coli |
More | enzyme exists in monomer, dimer, and higher oligomeric forms, which are associated with its regulation by allosteric effectors. In barbital buffer the enzyme is present as a monomer. In sodium phosphate buffer, the enzyme exists as a partially dissociating dimer. In presence of phosphate and a positive allosteric effector, the maximum association state of the enzyme is a tetramer. UMP promotes conversion to a dimer | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | enhances activity | Escherichia coli | |
GDP | enhances activity | Escherichia coli | |
GMP | enhances activity | Escherichia coli | |
IDP | enhances activity | Escherichia coli | |
IMP | phosphate decreases activation | Escherichia coli | |
IMP | stimulates | Escherichia coli |