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Literature summary for 6.3.5.4 extracted from

  • Reitzer, L.J.; Magasanik, B.
    Asparagine synthetases of Klebsiella aerogenes: properties and regulation of synthesis (1982), J. Bacteriol., 151, 1299-1313.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
5-Chloro-4-oxo-L-norvaline
-
Klebsiella aerogenes
6-diazo-5-oxo-L-norleucine
-
Klebsiella aerogenes
AMP
-
Klebsiella aerogenes
Asn
-
Klebsiella aerogenes
azaserine
-
Klebsiella aerogenes
diphosphate
-
Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information biphasic kinetic, linear portion near 0.5 Klebsiella aerogenes
0.24
-
ATP ATP Klebsiella aerogenes
0.55
-
Gln
-
Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
4 * 57000, denaturing PAGE Klebsiella aerogenes
230000
-
gel filtration Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella aerogenes Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting ?
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
the organism contains both enzymes EC 6.3.1.1. and EC 6.3.4.5. The gene asnA codes for NH4+-dependent Asn synthetases, EC 6.3.1.1, and the gene asnB codes for Gln-dependent Asn synthetase, EC 6.3.5.4
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + L-Gln
-
Klebsiella aerogenes AMP + diphosphate + Asn + Glu
-
?
ATP + L-Asp + NH3
-
Klebsiella aerogenes AMP + diphosphate + Asn
-
?
additional information Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting Klebsiella aerogenes ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57000, denaturing PAGE Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5 Gln-dependent activity Klebsiella aerogenes