Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Chloro-4-oxo-L-norvaline | - |
Klebsiella aerogenes | |
6-diazo-5-oxo-L-norleucine | - |
Klebsiella aerogenes | |
AMP | - |
Klebsiella aerogenes | |
Asn | - |
Klebsiella aerogenes | |
azaserine | - |
Klebsiella aerogenes | |
diphosphate | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | biphasic kinetic, linear portion near 0.5 | Klebsiella aerogenes | |
0.24 | - |
ATP | ATP | Klebsiella aerogenes | |
0.55 | - |
Gln | - |
Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
4 * 57000, denaturing PAGE | Klebsiella aerogenes |
230000 | - |
gel filtration | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella aerogenes | Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
the organism contains both enzymes EC 6.3.1.1. and EC 6.3.4.5. The gene asnA codes for NH4+-dependent Asn synthetases, EC 6.3.1.1, and the gene asnB codes for Gln-dependent Asn synthetase, EC 6.3.5.4 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + L-Gln | - |
Klebsiella aerogenes | AMP + diphosphate + Asn + Glu | - |
? | |
ATP + L-Asp + NH3 | - |
Klebsiella aerogenes | AMP + diphosphate + Asn | - |
? | |
additional information | Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting | Klebsiella aerogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57000, denaturing PAGE | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | Gln-dependent activity | Klebsiella aerogenes |