Cloned (Comment) | Organism |
---|---|
gene ASN3, real-time quantitative RT-PCR isozyme expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | phenotypes of isozyme ASN3 knockout mutant lines asn3-1 (SALK_053490) and asn3-2 (SALK_074279), The level of ASN3 mRNA is reduced to 2.5% and 15% of the wild-type value in the seeds of asn3-1 and asn3-2, respectively, overview. Impact of ASN3 disruption on asparagine, glutamine, aspartate and glutamate levels in asn3-1 siliques and compared to wild-type: The young siliques of the asn3-1 knockout line show an increase in glutamine (Glnasn3-1 to GlnCol-0 ratio of 1.014), glutamate (Gluasn3-1 to GluCol-0 ratio of 1.189) and aspartate (Aspasn3-1 to AspCol-0 ratio of 1.149) and a decrease in asparagine (Asnasn3-1 to AsnCol-0 ratio of 0.902) | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O | Arabidopsis thaliana | - |
AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + L-glutamine + H2O | Arabidopsis thaliana Col-0 | - |
AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LFU1 | - |
- |
Arabidopsis thaliana Col-0 | Q9LFU1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | rosette leaves, of 35-day-old plants | Arabidopsis thaliana | - |
silique | fourth siliques numbered from the top of 35-day-old plants | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O | - |
Arabidopsis thaliana | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + L-glutamine + H2O | - |
Arabidopsis thaliana Col-0 | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65200, about, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
ASN3 | - |
Arabidopsis thaliana |
At5g10240 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis has grouped ASN1 in dicot-subclass I while ASN2 and ASN3 are placed in dicot-subclass II | Arabidopsis thaliana |
malfunction | no visible phenotype is detected for the asn3-1 and asn3-2 mutant. Both asn3-1 and asn3-2 rosette leaves contain wild-type levels of chlorophyll and ammonium content, indicating that ASN3 disruption does not cause a defective nitrogen status during vegetative growth. During seed development, leaves and stems serve as source tissues to supply nitrogen resources to developing siliques which in turn deliver nitrogen to seeds. When compared to wild-type seeds, asn3-1 seeds display reduced glutamine (by 30%), asparagine (20%) and aspartate (20%) contents while exhibiting increased glutamate (10%) amounts | Arabidopsis thaliana |
physiological function | asparagine synthetase transfers the amide group of glutamine to aspartate, forming asparagine and glutamate. Asparagine, glutamine, aspartate and glutamate are important nitrogen carriers transported in the phloem, asparagine is a major nitrogen transporter since it contains more nitrogen per carbon (2N:4C) compared to glutamine (2N:5C), aspartate (1N:4C) and glutamate (1N:5C). Role of isozyme ASN3-encoded asparagine synthetase during vegetative growth, seed development and germination of Arabidopsis thaliana, overview | Arabidopsis thaliana |