Literature summary for 6.3.5.4 extracted from

Ciustea, M.; Gutierrez, J.A.; Abbatiello, S.E.; Eyler, J.R.; Richards, N.G.
Efficient expression, purification, and characterization of C-terminally tagged, recombinant human asparagine synthetase (2005), Arch. Biochem. Biophys., 440, 18-27.

Search for more literature for 6.3.5.4

Cloned(Commentary)

Cloned (Comment)	Organism
C-terminally tagged enzyme, baculovirus-based expression system, the recombinant enzyme is correctly processed, exhibits high activity and is stable on prolonged storage at -80°C	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	ATP	pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme	Homo sapiens
0.11	-	ATP	pH 8, reaction with NH3, C-terminally tagged recombinant enzyme	Homo sapiens
0.38	-	aspartic acid	pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme	Homo sapiens
1.3	-	L-aspartic acid	pH 8, reaction with NH3, C-terminally tagged recombinant enzyme	Homo sapiens
1.7	-	NH3	pH 8, C-terminally tagged recombinant enzyme	Homo sapiens
1.9	-	L-glutamine	pH 8, C-terminally tagged recombinant enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
T-cell	-	Homo sapiens	-

Storage Stability

Storage Stability	Organism
-80°C, recombinant C-terminally tagged enzyme is stable on prolonged storage	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-Asp + L-Gln	-	Homo sapiens	AMP + diphosphate + Asn + Glu	-	?
ATP + L-Asp + NH3	-	Homo sapiens	AMP + diphosphate + Asn	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	L-aspartic acid	pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme	Homo sapiens
1.6	-	ATP	pH 8, reaction with NH3, C-terminally tagged recombinant enzyme	Homo sapiens
1.7	-	glutamine	pH 8, C-terminally tagged recombinant enzyme	Homo sapiens
1.7	-	ATP	pH 8, reaction with glutamine, C-terminally tagged recombinant enzyme	Homo sapiens
1.7	-	L-aspartic acid	pH 8, reaction with NH3, C-terminally tagged recombinant enzyme	Homo sapiens
1.8	-	NH3	pH 8, C-terminally tagged recombinant enzyme	Homo sapiens

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Release 2023.1 (January 2023)