Cloned (Comment) | Organism |
---|---|
three asparagine snythetase genes asnB, asnH and asnO, expression in Bacillus subtilis and in Escherichia coli ME6279, sequencing, genetic organization | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | various mutant strains, deletion of asnO or asnH, singly or in combination, has no effect on growth rates in media with or without asparagine, deletion of asnB leads to a slow-growth phenotype, even in the presence of asparagine, strains lacking asnO cannot sporulate | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis | ? | - |
? | |
additional information | Bacillus subtilis 168 | physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
three glutamine-dependent AsnB-type asparagine snythetase genes: asnB, asnH and asnO, formerly yisO | - |
Bacillus subtilis 168 | - |
three glutamine-dependent AsnB-type asparagine snythetase genes: asnB, asnH and asnO, formerly yisO | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the three asparagine synthetase genes asnB, asnH and asnO are differentially expressed during cell growth, expression pattern | Bacillus subtilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | - |
Bacillus subtilis | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + L-glutamine | - |
Bacillus subtilis 168 | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
additional information | physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis | Bacillus subtilis | ? | - |
? | |
additional information | physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis | Bacillus subtilis 168 | ? | - |
? |