General Stability | Organism |
---|---|
enzyme requires protection by high levels of thiols, glycerol and substrates also stabilize | Lupinus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Lupinus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | MgATP2- | Lupinus luteus | |
0.16 | - |
Gln | - |
Lupinus luteus | |
1.3 | - |
Asp | - |
Lupinus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ cannot be replaced by Zn2+, Fe2+, Co2+, Ba2+ or Ca2+ | Lupinus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
substrate-free enzyme, gel filtration | Lupinus luteus |
320000 | - |
in presence of MgCl2 and ATP association to a dimer, gel filtration | Lupinus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lupinus luteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Lupinus luteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lupinus luteus |
Storage Stability | Organism |
---|---|
-25°C, 60% loss of activity after 3 months | Lupinus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + L-Gln | - |
Lupinus luteus | AMP + diphosphate + Asn + Glu | - |
? | |
additional information | ATP-diphosphate exchange reaction | Lupinus luteus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | dimerization of the 160000 MW enzyme is induced by MgCl2 and ATP | Lupinus luteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 90 s | Lupinus luteus |
56 | - |
half-life: 30 s | Lupinus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Lupinus luteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 8.3 | 80% of maximal activity at pH 6.9 and 8.3 | Lupinus luteus |