Cloned (Comment) | Organism |
---|---|
and overexpression | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C1A | replacement of Cys1 by either Ala or Ser results in a loss of glutaminase activity and Gln-dependent activity, without any significant effect upon NH4+-dependent Asn synthesis. Kinetic parameters of the NH4+-dependent activity of H29A and H80A are unchanged with respect to wild-type AS-B, the apparent Km for Gln is increased by a factor of 4.5 in Gln-dependent Asn synthesis | Escherichia coli |
C1S | replacement of Cys1 by either Ala or Ser results in a loss of glutaminase activity and Gln-dependent activity, without any significant effect upon NH4+-dependent Asn synthesis. Kinetic parameters of the NH4+-dependent activity of H29A and H80A are unchanged with respect to wild-type AS-B, the apparent Km for Gln is increased by a factor of 4.5 in Gln-dependent Asn synthesis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Gln | inhibitor of the NH4+-dependent reaction catalyzed by both the C1A and C1S mutants of AS-B | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
Asp | NH4+-dependent activity, wild-type | Escherichia coli | |
0.66 | - |
Gln | wild-type enzyme | Escherichia coli | |
0.85 | - |
Asp | Asp, wild-type enzyme, Gln-dependent activity | Escherichia coli | |
17 | - |
NH4+ | wild-type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | is the only divalent metal ion that can replace Mg2+ with 80% of the Mg2+-dependent AS-B activity activity with Gln and 52% of the Mg2+-dependent AS-B activity with NH4+ | Escherichia coli | |
Mg2+ | highest stimulation of divalent metal ions | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes C1A, C1S, H29A , H80A, D33N, D33E, and A104H | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + L-Gln | - |
Escherichia coli | AMP + diphosphate + Asn + Glu | - |
? | |
CTP + L-Asp + L-Gln | weak activity | Escherichia coli | CMP + diphosphate + Asn + Glu | - |
? | |
dATP + L-Asp + L-Gln | utilized at a similar rate as ATP | Escherichia coli | dAMP + diphosphate + Asn + Glu | - |
? | |
dATP + L-Asp + NH3 | utilized at a similar rate as ATP | Escherichia coli | dAMP + diphosphate + Asn | - |
? | |
GTP + L-Asp + L-Gln | 15% of the activity relative to ATP | Escherichia coli | GMP + diphosphate + Asn + Glu | - |
? | |
additional information | no activity with ITP or XTP | Escherichia coli | ? | - |
? | |
UTP + L-Asp + L-Gln | weak activity | Escherichia coli | UMP + diphosphate + Asn + Glu | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Escherichia coli |