Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21DE3-834 (gold) cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
mutants R1263A and F209W, vapor diffusion method | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
F209W | both the secondary structure content and activity of the mutant enzyme is very similar to the wild type protein | Salmonella enterica subsp. enterica serovar Typhimurium |
L1181F | the mutation causes a 60% loss in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
L1181W | the mutant has secondary structure content and enzyme at par with the wild type protein and exhibits almost no destabilization | Salmonella enterica subsp. enterica serovar Typhimurium |
L1181Y | the mutation results in failure to yield soluble protein | Salmonella enterica subsp. enterica serovar Typhimurium |
R1263A | the mutant is structurally identical to the the native protein | Salmonella enterica subsp. enterica serovar Typhimurium |
T683W | the mutation causes a 70% loss in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | P74881 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FGAM synthetase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
FGAR-AT | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
formylglycinamide ribonucleotide amidotransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
formylglycinamide synthetase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
PurL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | 80 | the first unfolding transition at 42°C leads to a loss of 15-20% of the secondary structure content of the protein and 80% loss in activity. The second unfolding transition at 80°C represents unfolding of the bulk of the protein | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |