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Literature summary for 6.3.5.3 extracted from

  • Anand, R.; Hoskins, A.A.; Bennett, E.M.; Sintchak, M.D.; Stubbe, J.; Ealick, S.E.
    A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex (2004), Biochemistry, 43, 10343-10352.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli B834(DE3) Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
determination of structure of PurS in two different crystal forms P2(1) and C2 at 2.5 and 2.0 A resolution, respectively. PurS forms a tight dimer with a central six-stranded beta-sheet flanked by four helices. In both the P2(1) and the C2 crystal forms, the quaternary structure of PurS is a tetramer. Native PurS is crystallized from 2.0 M ammonium sulfate, 6% PEG 400, 100 mM HEPES, pH 7.3. The crystals are grown at room temperature using the hanging-drop vapor diffusion technique. Drops containing a 2:1 mixture of protein and reservoir solution are optimal for crystal growth. Crystals grow over a period of 4-7 days. Under these conditions, PurS crystallizes in the monoclinic space group P2(1) with unit cell dimensions a = 42.91 A, b = 87.96 A, c = 52.68 A, and beta = 94.97°. The SeMet-PurS crystals grow in 20% PEG 4K, 5% glycerol, 15% 2-propanol, 100 mM sodium citrate, pH 5.6. Optimal drop size is 0.004 mL using a 1:1 mixture of protein and reservoir. Under these conditions, the protein crystallizes in the space group C2 with unit cell dimensions a = 89.13 A, b = 42.21 A, c = 47.03 A, and beta = 118.22° Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
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PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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-
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Purification (Commentary)

Purification (Comment) Organism
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Bacillus subtilis

Synonyms

Synonyms Comment Organism
mPurL PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity Bacillus subtilis
PurL
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Bacillus subtilis
PurL PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity Bacillus subtilis
smPurL PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity Bacillus subtilis