Cloned (Comment) | Organism |
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expression in Escherichia coli B834(DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
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determination of structure of PurS in two different crystal forms P2(1) and C2 at 2.5 and 2.0 A resolution, respectively. PurS forms a tight dimer with a central six-stranded beta-sheet flanked by four helices. In both the P2(1) and the C2 crystal forms, the quaternary structure of PurS is a tetramer. Native PurS is crystallized from 2.0 M ammonium sulfate, 6% PEG 400, 100 mM HEPES, pH 7.3. The crystals are grown at room temperature using the hanging-drop vapor diffusion technique. Drops containing a 2:1 mixture of protein and reservoir solution are optimal for crystal growth. Crystals grow over a period of 4-7 days. Under these conditions, PurS crystallizes in the monoclinic space group P2(1) with unit cell dimensions a = 42.91 A, b = 87.96 A, c = 52.68 A, and beta = 94.97°. The SeMet-PurS crystals grow in 20% PEG 4K, 5% glycerol, 15% 2-propanol, 100 mM sodium citrate, pH 5.6. Optimal drop size is 0.004 mL using a 1:1 mixture of protein and reservoir. Under these conditions, the protein crystallizes in the space group C2 with unit cell dimensions a = 89.13 A, b = 42.21 A, c = 47.03 A, and beta = 118.22° | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
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additional information | - |
PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
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Bacillus subtilis | - |
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Purification (Comment) | Organism |
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- |
Bacillus subtilis |
Synonyms | Comment | Organism |
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mPurL | PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity | Bacillus subtilis |
PurL | - |
Bacillus subtilis |
PurL | PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity | Bacillus subtilis |
smPurL | PurL exists in two forms: large PurL (lgPurL) is a single chain, multidomain enzyme of about 1300 amino acids, whereas small PurL (smPurL) contains about 800 amino acids but requires two additional gene products, PurS and PurQ, for activity | Bacillus subtilis |