Cloned (Comment) | Organism |
---|---|
MTH169 gene amplified by PCR using Mth genomic DNA as template, cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3) | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
crucial component MTH169, space group P4(3)22, unit cell parameters a : 53.8 A, c : 142.7 A | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-phosphoribosylformylglycinamide + L-glutamine + ATP | Methanothermobacter thermautotrophicus | fourth step in the de novo purine biosynthetic pathway | phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
thermophilic archaeon | - |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-phosphoribosylformylglycinamide + L-glutamine + ATP | - |
Methanothermobacter thermautotrophicus | phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate | - |
r | |
5'-phosphoribosylformylglycinamide + L-glutamine + ATP | fourth step in the de novo purine biosynthetic pathway | Methanothermobacter thermautotrophicus | phosphoribosylformylglycinamidine + L-glutamate + ADP + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
FGAM synthetase | - |
Methanothermobacter thermautotrophicus |
Phosphoribosylformylglycinamidine synthetase | - |
Methanothermobacter thermautotrophicus |