Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.2 extracted from

  • van der Knaap, J.A.; Kozhevnikova, E.; Langenberg, K.; Moshkin, Y.M.; Verrijzer, C.P.
    Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes (2010), Mol. Cell. Biol., 30, 736-744.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.2

Cloned(Commentary)

Cloned (Comment) Organism
the Ecdysone receptor interacts biochemically and genetically with GMPS/USP7, co-expression of GST-tagged GMPS and USP7 in Escherichia coli strain BL21(DE3) Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
C95A mutant defective in glutamine hydrolysis. The phenotype of ectopic coexpression of USP7 witheither S242L or C95A is similar to that resulting from the coexpression of WT GMPS. Ectopic overexpression of only mutation S242L, mutation C95A, or USP7 mutation C250A has no effect on eye development Drosophila melanogaster
S242L mutant defective in ATP hydrolysis. The phenotype of ectopic coexpression of USP7 with either S242L or C95A is similar to that resulting from the coexpression of WT GMPS. Ectopic overexpression of only mutation S242L, mutation C95A, or USP7 mutation C250A has no effect on eye development Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information binding dynamics at ecdysone target loci, overview Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Drosophila melanogaster 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster GMP synthetase binds ubiquitin-specific protease 7, USP7, and deubiquitylates histone H2B, the GMPS/USP7 complex cooperates with the Polycomb silencing system through removal of the active ubiquitin mark from histone H2B. GMPS/USP7 binds ecdysone-regulated loci prior to or after hormone signaling. The Ecdysone receptor interacts biochemically and genetically with GMPS/USP7 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information GMP synthetase binds ubiquitin-specific protease 7, USP7, and deubiquitylates histone H2B, the GMPS/USP7 complex cooperates with the Polycomb silencing system through removal of the active ubiquitin mark from histone H2B. GMPS/USP7 binds ecdysone-regulated loci prior to or after hormone signaling. The Ecdysone receptor interacts biochemically and genetically with GMPS/USP7 Drosophila melanogaster ?
-
 ?

Synonyms

Synonyms Comment Organism
GMP synthetase
-
 Drosophila melanogaster
GMPS
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Drosophila melanogaster

General Information

General Information Comment Organism
malfunction GMPS/USP7 complex mutants display severe misregulation of ecdysone target genes Drosophila melanogaster
physiological function GMPS is required for its ability to deubiquitylate histone H2B. A key enzyme in the guanine nucleotide biosynthesis pathway is involved in nuclear receptor target silencing suggests that GMPS might provide a relay between metabolic state and developmental gene switching Drosophila melanogaster
physiological function GMP synthetase binds ubiquitin-specific protease USP7 and is required for its ability to deubiquitylate histone H2B. Strong cooperation between GMPS and USP7, which is counteracted by the histone H2B ubiquitin ligase BRE1. Loss of either GMPS or USP7 leads to increased levels of histone H2Bub in mutant animals. GMPS/USP7 binds ecdysone-regulated loci and mutants display severe misregulation of ecdysone target genes. Ecdysone receptor EcR interacts biochemically and genetically with GMPS/USP7. Analyses suggest that GMPS/USP7 acts as a transcriptional corepressor Drosophila melanogaster