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Literature summary for 6.3.5.2 extracted from
- Studies with GMP synthetase from Ehrlich ascites cells. Purification, properties, and interactions with nucleotide analogs (1975), J. Biol. Chem., 250, 7372-7376.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | stimulates | Mus musculus |  |
General Stability
| General Stability | Organism |
|---|---|
| thawing and refreezing does not diminish activity | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Mus musculus | |
| 6-thioXMP | - |
Mus musculus | |
| hydroxylamine | - |
Mus musculus | |
| p-chloromercuribenzoate | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
XMP | - |
Mus musculus | |
| 0.05 | - |
8-azaXMP | - |
Mus musculus | |
| 0.125 | - |
2-dXMP | - |
Mus musculus | |
| 0.28 | - |
ATP | - |
Mus musculus | |
| 0.68 | - |
Gln | - |
Mus musculus | |
| 36 | - |
NH4+ | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0428 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, quite stable | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine 5-phosphate | - |
Mus musculus | AMP + phosphate + 1-ribosyl-4-hydroxy-6-aminopyrazolo[3,4-d]pyrimidine 5'-phosphate | - |
? | |
| ATP + 6-thioXMP + NH4+ | - |
Mus musculus | AMP + phosphate + 6-thioGMP | - |
? | |
| ATP + 8-azaXMP + NH4+ | - |
Mus musculus | AMP + phosphate + 8-azaGMP | - |
? | |
| ATP + beta-arabinofuranosyl-XMP | - |
Mus musculus | AMP + phosphate + beta-arabinofuranosylGMP | - |
? | |
| ATP + dXMP + NH4+ | - |
Mus musculus | AMP + phosphate + dGMP | - |
? | |
| ATP + XMP + Gln | higher Km value for NH4+ compared with Gln | Mus musculus | AMP + diphosphate + GMP + Glu | - |
? | |
| ATP + XMP + NH4+ | higher Km value for NH4+ compared with Gln | Mus musculus | AMP + diphosphate + GMP | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8.6 | more than 80% of maximal activity at pH 7.4 and 8.6 | Mus musculus |
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