Activating Compound | Comment | Organism | Structure |
---|---|---|---|
reduced sulfhydryl compounds | e.g. dithiothreitol, required | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Rattus norvegicus | |
cAMP | - |
Rattus norvegicus | |
cGMP | - |
Rattus norvegicus | |
CMP | - |
Rattus norvegicus | |
GMP | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
XMP | - |
Rattus norvegicus | |
0.12 | - |
ATP | - |
Rattus norvegicus | |
0.3 | - |
Gln | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | Yoshida sarcoma ascites cells | Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | high activity only in presence of Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Yoshida sarcoma ascites cells | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
intestine | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
ovary | - |
Rattus norvegicus | - |
skeletal muscle | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
Yoshida ascites sarcoma cell | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + XMP + Gln | maximal activity with NH4+ is 80% of that obtained with Gln | Rattus norvegicus | AMP + diphosphate + GMP + Glu | - |
? | |
ATP + XMP + Gln | higher Km value for NH4+ compared with Gln | Rattus norvegicus | AMP + diphosphate + GMP + Glu | - |
? | |
ATP + XMP + NH4+ | maximal activity with NH4+ is 80% of that obtained with Gln | Rattus norvegicus | AMP + diphosphate + GMP | - |
? | |
ATP + XMP + NH4+ | higher Km value for NH4+ compared with Gln | Rattus norvegicus | AMP + diphosphate + GMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | more than 50% of maximal activity at pH 6.5 and pH 9.0 | Rattus norvegicus |