BRENDA - Enzyme Database
show all sequences of 6.3.5.10

The multiple amidation reactions catalyzed by cobyric acid synthetase from Salmonella typhimurium are sequential and dissociative

Williams, L.; Fresquet, V.; Santander, P.J.; Raushel, F.M.; J. Am. Chem. Soc. 129, 294-295 (2007)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cobyric acid
-
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0007
-
adenosylcobyrinic acid pentaamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
0.0008
-
adenosylcobyrinic acid tetraamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
0.001
-
adenosylcobyrinic acid triamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
0.0027
-
adenosylcobyrinic acid diamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + adenosylcobyrinic acid diamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid pentaamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid tetraamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid triamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4
-
adenosylcobyrinic acid triamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
3.6
-
adenosylcobyrinic acid diamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
3.6
-
adenosylcobyrinic acid tetraamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
8.4
-
adenosylcobyrinic acid pentaamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0059
-
cobyric acid
-
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cobyric acid
-
Salmonella enterica subsp. enterica serovar Typhimurium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0059
-
cobyric acid
-
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0007
-
adenosylcobyrinic acid pentaamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
0.0008
-
adenosylcobyrinic acid tetraamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
0.001
-
adenosylcobyrinic acid triamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
0.0027
-
adenosylcobyrinic acid diamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + adenosylcobyrinic acid diamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid pentaamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid tetraamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid triamide + L-glutamine + H2O
four carboxylates attached to the corrinoid ring are amidated in a dissociative fashion. The four carboxylates are amidated in a sequence beginning with carboxylate e and followed in turn by carboxylates d, b, and g
674206
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4
-
adenosylcobyrinic acid triamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
3.6
-
adenosylcobyrinic acid diamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
3.6
-
adenosylcobyrinic acid tetraamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
8.4
-
adenosylcobyrinic acid pentaamide
30°C, pH 7.5
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 6.3.5.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674206
Williams
The multiple amidation reactio ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Am. Chem. Soc.
129
294-295
2007
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1
4
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2
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4
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4
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1
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1
1
4
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4
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4
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655868
Woodson
A new pathway for salvaging th ...
Halobacterium sp., Salmonella enterica, Salmonella enterica TR6583
J. Bacteriol.
185
7193-7201
2003
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2
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2
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4
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3
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8
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2
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2
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4
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8
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644278
Warren
The biosynthesis of adenosylco ...
Pseudomonas denitrificans (nomen rejiciendum), Salmonella enterica
Nat. Prod. Rep.
19
390-412
2002
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1
2
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2
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4
1
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1
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1
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1
2
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4
1
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653832
Galperin
The synthetase domains of coba ...
Escherichia coli, Pseudomonas denitrificans (nomen rejiciendum)
Proteins
41
238-247
2000
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-
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-
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1
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1
2
2
-
5
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1
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4
2
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-
-
-
2
-
-
1
-
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-
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-
1
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-
1
-
1
2
2
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4
2
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2
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-
-
644281
Blanche F.; Maton
Purification and characterizat ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
174
7452-7454
1992
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1
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1
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1
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1
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1
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636869
Crouzet
Nucleotide sequence and geneti ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 Rifr(pXL617)
J. Bacteriol.
173
6074-6087
1991
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1
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2
2
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4
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1
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4
1
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1
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2
2
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1
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4
1
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-
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651618
Blanche
Biosynthesis of vitamin B12: s ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6046-6051
1991
-
-
1
-
-
1
-
14
-
1
3
2
-
1
1
-
1
-
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-
1
2
14
2
-
-
-
-
1
-
-
1
-
1
-
-
-
1
1
-
-
1
-
-
-
14
-
1
3
2
-
1
-
1
-
-
1
2
14
2
-
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-
1
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1
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