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Literature summary for 6.3.5.10 extracted from

  • Galperin, M.Y.; Grishin, N.V.
    The synthetase domains of cobalamin biosynthesis amidotransferases cobB and cobQ belong to a new family of ATP-dependent amidoligases, related to dethiobiotin synthetase (2000), Proteins, 41, 238-247.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ bound Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 2 * 57000 Escherichia coli
57000
-
 2 * 57000 Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O Escherichia coli vitamin B12 biosynthesis adenosylcobyric acid + ADP + phosphate + L-glutamate
-
 ?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O Pseudomonas denitrificans (nom. rej.) involved in cobalamin biosynthesis ADP + phosphate + adenosylcobyric acid + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas denitrificans (nom. rej.)
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate catalytic mechanism Pseudomonas denitrificans (nom. rej.)
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
-
 Escherichia coli adenosylcobyric acid + ADP + phosphate + L-glutamate
-
 ?
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O vitamin B12 biosynthesis Escherichia coli adenosylcobyric acid + ADP + phosphate + L-glutamate
-
 ?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O involved in cobalamin biosynthesis Pseudomonas denitrificans (nom. rej.) ADP + phosphate + adenosylcobyric acid + L-glutamate
-
 ?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O CobQ contains an unusual Triad family, class I, glutamine amidotransferase domain with conserved Cys and His residues, but lacking the Glu residue of the catalytic triad, domain organization, model of substrate binding, catalytic mechanism, CobQ catalyzes amidation of the carboxyls b, d, e, and g in a strict order, one ATP molecule and one glutamine molecule are consumed for each amidation reaction Pseudomonas denitrificans (nom. rej.) ADP + phosphate + adenosylcobyric acid + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57000 Escherichia coli
dimer 2 * 57000 Pseudomonas denitrificans (nom. rej.)

Synonyms

Synonyms Comment Organism
cobalamin biosynthesis aminotransferase
-
 Escherichia coli
CobQ
-
 Escherichia coli
cobyric acid synthase
-
 Escherichia coli
More CobQ is a member of the dethiobiotin synthetase superfamily Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli
7.5
-
-
 Pseudomonas denitrificans (nom. rej.)

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent Pseudomonas denitrificans (nom. rej.)