BRENDA - Enzyme Database show
show all sequences of 6.3.5.10

The synthetase domains of cobalamin biosynthesis amidotransferases cobB and cobQ belong to a new family of ATP-dependent amidoligases, related to dethiobiotin synthetase

Galperin, M.Y.; Grishin, N.V.; Proteins 41, 238-247 (2000)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
bound
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
2 * 57000
Escherichia coli
57000
-
2 * 57000
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
Escherichia coli
vitamin B12 biosynthesis
adenosylcobyric acid + ADP + phosphate + L-glutamate
-
Escherichia coli
?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
involved in cobalamin biosynthesis
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Pseudomonas denitrificans (nomen rejiciendum)
-
-
-
Reaction
Reaction
Commentary
Organism
4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate
catalytic mechanism
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
-
653832
Escherichia coli
adenosylcobyric acid + ADP + phosphate + L-glutamate
-
653832
Escherichia coli
?
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
vitamin B12 biosynthesis
653832
Escherichia coli
adenosylcobyric acid + ADP + phosphate + L-glutamate
-
653832
Escherichia coli
?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
involved in cobalamin biosynthesis
653832
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
CobQ contains an unusual Triad family, class I, glutamine amidotransferase domain with conserved Cys and His residues, but lacking the Glu residue of the catalytic triad, domain organization, model of substrate binding, catalytic mechanism, CobQ catalyzes amidation of the carboxyls b, d, e, and g in a strict order, one ATP molecule and one glutamine molecule are consumed for each amidation reaction
653832
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 57000
Escherichia coli
dimer
2 * 57000
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Escherichia coli
7.5
-
-
Pseudomonas denitrificans (nomen rejiciendum)
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
ATP-dependent
Pseudomonas denitrificans (nomen rejiciendum)
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
ATP-dependent
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
bound
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
2 * 57000
Escherichia coli
57000
-
2 * 57000
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
Escherichia coli
vitamin B12 biosynthesis
adenosylcobyric acid + ADP + phosphate + L-glutamate
-
Escherichia coli
?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
involved in cobalamin biosynthesis
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
-
653832
Escherichia coli
adenosylcobyric acid + ADP + phosphate + L-glutamate
-
653832
Escherichia coli
?
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O
vitamin B12 biosynthesis
653832
Escherichia coli
adenosylcobyric acid + ADP + phosphate + L-glutamate
-
653832
Escherichia coli
?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
involved in cobalamin biosynthesis
653832
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O
CobQ contains an unusual Triad family, class I, glutamine amidotransferase domain with conserved Cys and His residues, but lacking the Glu residue of the catalytic triad, domain organization, model of substrate binding, catalytic mechanism, CobQ catalyzes amidation of the carboxyls b, d, e, and g in a strict order, one ATP molecule and one glutamine molecule are consumed for each amidation reaction
653832
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + adenosylcobyric acid + L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 57000
Escherichia coli
dimer
2 * 57000
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Escherichia coli
7.5
-
-
Pseudomonas denitrificans (nomen rejiciendum)
Other publictions for EC 6.3.5.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674206
Williams
The multiple amidation reactio ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Am. Chem. Soc.
129
294-295
2007
-
-
-
-
-
-
1
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
655868
Woodson
A new pathway for salvaging th ...
Halobacterium sp., Salmonella enterica, Salmonella enterica TR6583
J. Bacteriol.
185
7193-7201
2003
-
-
2
-
2
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644278
Warren
The biosynthesis of adenosylco ...
Pseudomonas denitrificans (nomen rejiciendum), Salmonella enterica
Nat. Prod. Rep.
19
390-412
2002
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653832
Galperin
The synthetase domains of coba ...
Escherichia coli, Pseudomonas denitrificans (nomen rejiciendum)
Proteins
41
238-247
2000
-
-
-
-
-
-
-
1
-
1
2
2
-
5
-
-
-
1
-
-
-
-
4
2
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
2
2
-
-
-
-
-
-
-
-
4
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
644281
Blanche F.; Maton
Purification and characterizat ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
174
7452-7454
1992
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636869
Crouzet
Nucleotide sequence and geneti ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 Rifr(pXL617)
J. Bacteriol.
173
6074-6087
1991
-
-
1
-
-
-
-
-
-
-
2
2
-
4
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651618
Blanche
Biosynthesis of vitamin B12: s ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6046-6051
1991
-
-
1
-
-
1
-
14
-
1
3
2
-
1
1
-
1
-
-
-
1
2
14
2
-
-
-
-
1
-
-
1
-
1
-
-
-
1
1
-
-
1
-
-
-
14
-
1
3
2
-
1
-
1
-
-
1
2
14
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-