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Literature summary for 6.3.5.1 extracted from

  • Laskoski, K.; Santos, A.R.; Bonatto, A.C.; Pedrosa, F.O.; Souza, E.M.; Huergo, L.F.
    In vitro characterization of the NAD+ synthetase NadE1 from Herbaspirillum seropedicae (2016), Arch. Microbiol., 198, 307-313 .
    View publication on PubMed
Search for more literature for 6.3.5.1

Cloned(Commentary)

Cloned (Comment) Organism
gene nadE1, sequence comparisons, the nadE1 gene is linked to glnB encoding the signal transduction protein GlnB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Herbaspirillum seropedicae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten and Hanes-Woolf kinetics, kinetic analysis Herbaspirillum seropedicae
0.01
-
 deamido-NAD+ pH 8.5, 30°C, recombinant His-tagged enzyme Herbaspirillum seropedicae
0.031
-
 ATP pH 8.5, 30°C, recombinant His-tagged enzyme Herbaspirillum seropedicae
0.365
-
 L-glutamine pH 8.5, 30°C, recombinant His-tagged enzyme Herbaspirillum seropedicae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Herbaspirillum seropedicae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
548000
-
 recombinant enzyme, gel filtration Herbaspirillum seropedicae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O Herbaspirillum seropedicae
-
 AMP + diphosphate + NAD+ + L-glutamate
-
 ?
ATP + deamido-NAD+ + L-glutamine + H2O Herbaspirillum seropedicae SmR1
-
 AMP + diphosphate + NAD+ + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Herbaspirillum seropedicae D8IS59
-
-
Herbaspirillum seropedicae SmR1 D8IS59
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Herbaspirillum seropedicae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O
-
 Herbaspirillum seropedicae AMP + diphosphate + NAD+ + L-glutamate
-
 ?
ATP + deamido-NAD+ + L-glutamine + H2O
-
 Herbaspirillum seropedicae SmR1 AMP + diphosphate + NAD+ + L-glutamate
-
 ?
additional information enzyme NadE1 uses glutamine as a preferential nitrogen donor, poor activity with NH3 Herbaspirillum seropedicae ?
-
 ?
additional information enzyme NadE1 uses glutamine as a preferential nitrogen donor, poor activity with NH3 Herbaspirillum seropedicae SmR1 ?
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 70000-75000, recombinant enzyme, SDS-PAGE, sequence calculation, and gel filtration Herbaspirillum seropedicae

Synonyms

Synonyms Comment Organism
glutamine-dependent NAD synthetase
-
 Herbaspirillum seropedicae
glutamine-dependent NadEGln
-
 Herbaspirillum seropedicae
Hsero_1894
-
 Herbaspirillum seropedicae
NadE1
-
 Herbaspirillum seropedicae
nicotinamide adenine dinucleotide synthetase enzyme
-
 Herbaspirillum seropedicae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Herbaspirillum seropedicae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Herbaspirillum seropedicae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Herbaspirillum seropedicae