Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, with SUMO tag | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
deamido-NAD+ | pH 8.3, 37°C | Thermotoga maritima | |
0.095 | - |
ATP | pH 8.3, 37°C | Thermotoga maritima | |
0.33 | - |
NH3 | pH 8.3, 37°C | Thermotoga maritima | |
0.42 | - |
L-Gln | pH 8.3, 37°C | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-Gln | - |
Thermotoga maritima | AMP + diphosphate + NAD+ + L-Glu | - |
? | |
ATP + deamido-NAD+ + NH3 + H2O | - |
Thermotoga maritima | AMP + diphosphate + NAD+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
at temperatures above 65°C the glutamine-dependent reaction becomes about 20% faster than the ammonia-dependent at 80°C | Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 80 | at temperatures above 65°C the glutamine-dependent reaction becomes about 20% faster than the ammonia-dependent at 80°C | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
ATP | pH 8.3, 37°C | Thermotoga maritima | |
0.16 | - |
deamido-NAD+ | pH 8.3, 37°C | Thermotoga maritima | |
0.24 | - |
NH3 | pH 8.3, 37°C | Thermotoga maritima | |
0.288 | - |
L-Gln | pH 8.3, 37°C | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
L-Gln | pH 8.3, 37°C | Thermotoga maritima | |
0.7 | - |
NH3 | pH 8.3, 37°C | Thermotoga maritima | |
1.6 | - |
ATP | pH 8.3, 37°C | Thermotoga maritima | |
7.6 | - |
deamido-NAD+ | pH 8.3, 37°C | Thermotoga maritima |