Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C176A | complete loss of glutamine-dependent activity, retains 90% of its NH3-dependent activity | Mycobacterium tuberculosis |
E52A | complete loss of glutamine-dependent activity, retains 30% of its NH3-dependent activity | Mycobacterium tuberculosis |
K121A | complete loss of glutamine-dependent and NH3-dependent activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
deamido-NAD+ | wild-type enzyme | Mycobacterium tuberculosis | |
0.8 | - |
ATP | wild-type enzyme | Mycobacterium tuberculosis | |
0.8 | - |
ATP | mutant enzyme E52A | Mycobacterium tuberculosis | |
1.3 | - |
ATP | mutant enzyme C176A | Mycobacterium tuberculosis | |
1.4 | - |
deamido-NAD+ | mutant enzyme C176A | Mycobacterium tuberculosis | |
1.4 | - |
deamido-NAD+ | mutant enzyme E52A | Mycobacterium tuberculosis | |
1.6 | - |
L-Gln | wild-type enzyme | Mycobacterium tuberculosis | |
1.6 | - |
NH4+ | mutant enzyme E52A | Mycobacterium tuberculosis | |
2.3 | - |
NH4+ | mutant enzyme C176A | Mycobacterium tuberculosis | |
2.7 | - |
NH4+ | wild-type enzyme | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-Gln | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ + L-Glu | - |
? | |
ATP + deamido-NAD+ + NH4+ | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD+ synthetase | - |
Mycobacterium tuberculosis |