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Literature summary for 6.3.5.1 extracted from

  • Bembenek, M.E.; Kuhn, E.; Mallender, W.D.; Pullen, L.; Li, P.; Parsons, T.
    A fluorescence-based coupling reaction for monitoring the activity of recombinant human NAD synthetase (2005), Assay Drug Dev. Technol., 3, 533-541.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
gossypol
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
L-Gln pH 7.5 Homo sapiens
0.015
-
ATP pH 7.5 Homo sapiens
0.04
-
deamido-NAD+ pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant enzyme
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
coupled enzyme assay for the measurement of recombinant human NAD+ synthetase by employing lactate dehydrogenase in a cycling/amplification reaction linked ultimately to the fluorescence generation of resorufin from reazurin via diaphorase Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-Gln
-
Homo sapiens AMP + diphosphate + NAD+ + L-Glu
-
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