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Literature summary for 6.3.5.1 extracted from

  • Bellinzoni, M.; De Rossi, E.; Branzoni, M.; Milano, A.; Peverali, F.A.; Rizzi, M.; Riccardi, G.
    Heterologous expression, purification, and enzymatic activity of Mycobacterium tuberculosis NAD(+) synthetase (2002), Protein Expr. Purif., 25, 547-557.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
most efficient expression of His-tagged NadE-738 and NadE-679 in Escherichia coli strain Origami Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
deamido-NAD+ 37┬░C, pH 8.0, recombinant NadE-679 Mycobacterium tuberculosis
0.79
-
ATP 37┬░C, pH 8.0, recombinant NadE-679 Mycobacterium tuberculosis
1.1
-
L-glutamine 37┬░C, pH 8.8, recombinant NadE-738 Mycobacterium tuberculosis
1.3
-
ATP 37┬░C, pH 8.5, recombinant NadE-738 Mycobacterium tuberculosis
1.6
-
L-glutamine 37┬░C, pH 8.5, recombinant NadE-679 Mycobacterium tuberculosis
2.5
-
NH4+ 37┬░C, pH 8.0, recombinant NadE-679 Mycobacterium tuberculosis
2.9
-
deamido-NAD+ 37┬░C, pH 8.5, recombinant NadE-738 Mycobacterium tuberculosis
27
-
NH4+ 37┬░C, pH 8.5, recombinant NadE-738 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
8 * 67000, SDS-PAGE Mycobacterium tuberculosis
600000
-
gel filtration, NadE-679 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O Mycobacterium tuberculosis
-
AMP + diphosphate + NAD+ + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant NadE-738 and NadE-679, Ni2+-nitrilotriacetic acid agarose, gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.033
-
recombinant NadE-738, nitrogen donor glutamine Mycobacterium tuberculosis
0.1
-
recombinant NadE-738, nitrogen donor NH4+ Mycobacterium tuberculosis
0.44
-
recombinant NadE-679, nitrogen donor glutamine Mycobacterium tuberculosis
0.67
-
recombinant NadE-679, nitrogen donor NH4+ Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O
-
Mycobacterium tuberculosis AMP + diphosphate + NAD+ + L-glutamate
-
?
ATP + deamido-NAD+ + NH4+ + H2O
-
Mycobacterium tuberculosis AMP + diphosphate + NAD+
-
?

Subunits

Subunits Comment Organism
octamer 8 * 67000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
NadE-679 short NadE form, probably raising from translation of NadE gene from an internal start codon Mycobacterium tuberculosis
NadE-679
-
Mycobacterium tuberculosis
NadE-738
-
Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant NadE-679, nitrogen donor NH4+ Mycobacterium tuberculosis
8.5
-
recombinant NadE-738, nitrogen donor NH4+, recombinant NadE-679, nitrogen donor glutamine Mycobacterium tuberculosis
8.8
-
recombinant NadE-738, nitrogen donor glutamine Mycobacterium tuberculosis