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Literature summary for 6.3.5.1 extracted from

  • Hara, N.; Yamada, K.; Terashima, M.; Osago, H.; Shimoyama, M.; Tsuchiya, M.
    Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency (2003), J. Biol. Chem., 278, 10914-10921.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Nadsyn1 and mutant enzyme C175S as His6-tagged proteins in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C175S in contrast with the wild-type NADsyn1, the activity of the mutant NADsyn1 (C175S-NADsyn1) is not detected when glutamine is used as a substrate, whereas the activity remains unaltered with NH4Cl Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
deamido-NAD+ 37┬░C, wild-type NADsyn1 Homo sapiens
0.089
-
ATP 37┬░C, wild-type NADsyn1 Homo sapiens
0.12
-
ATP 37┬░C, NADsyn1 C175S mutant Homo sapiens
0.2
-
deamido-NAD+ 37┬░C, NADsyn1 C175S mutant Homo sapiens
1.44
-
L-glutamine 37┬░C, wild-type NADsyn1 Homo sapiens
13.1
-
NH3 37┬░C, wild-type NADsyn1 Homo sapiens
23.9
-
NH3 37┬░C, NADsyn1 C175S mutant Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
6 * 80000, wild-type and C175S mutant enzyme, SDS-PAGE Homo sapiens
80300
-
6 * 80300, calculated from sequence Homo sapiens
500000
-
non-denaturing PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6IA69
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
heart the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
kidney the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
liver the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
lung the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
skeletal muscle the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
small intestine the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
spleen the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-
testis the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O NADsyn1 uses not only ammonia but also glutamine. The Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3 Homo sapiens AMP + diphosphate + NAD+ + L-glutamate
-
?
ATP + deamido-NAD+ + NH3 NADsyn1 uses not only ammonia but also glutamine. The Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3 Homo sapiens AMP + diphosphate + NAD+
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 80000, wild-type and C175S mutant enzyme, SDS-PAGE Homo sapiens
hexamer 6 * 80300, calculated from sequence Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information the Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3 Homo sapiens