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Literature summary for 6.3.5.1 extracted from

  • Cantoni, R.; Branzoni, M.; Labo, M.; Rizzi, M.; Riccardi, G.
    The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase (1998), J. Bacteriol., 180, 3218-3221.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged NAD+ synthase in Escherichia coli Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
x * 98000, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O Mycobacterium tuberculosis last step in NAD+ biosynthesis AMP + diphosphate + NAD+ + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
NAD+ synthase is encoded by the MTCY428.08 gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant NAD+ synthase, Ni2+ chelate resin affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.09
-
glutamine-dependent activity Mycobacterium tuberculosis
0.23
-
NH3 dependent activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-glutamine + H2O
-
Mycobacterium tuberculosis AMP + diphosphate + NAD+ + L-glutamate
-
?
ATP + deamido-NAD+ + L-glutamine + H2O last step in NAD+ biosynthesis Mycobacterium tuberculosis AMP + diphosphate + NAD+ + L-glutamate
-
?
ATP + deamido-NAD+ + NH3 + H2O NAD+ synthase is able to use ammonia or glutamine as nitrogen source Mycobacterium tuberculosis AMP + diphosphate + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE Mycobacterium tuberculosis