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Literature summary for 6.3.5.1 extracted from

  • Zerez, C.R.; Wong, M.D.; Tanaka, K.R.
    Partial purification and properties of nicotinamide adenine dinucleotide synthetase from human erythrocytes: evidence that enzyme activity is a sensitive indicator of lead exposure (1990), Blood, 75, 1576-1582.
    View publication on PubMed

Application

Application Comment Organism
medicine erythrocyte NAD synthetase is activated by lead, and the activity is a sensitive indicator of lead exposure in human Homo sapiens

General Stability

General Stability Organism
EDTA and KCl increase stability substantially Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Pb2+
-
Homo sapiens
Zn2+
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.108
-
deamido-NAD+
-
Homo sapiens
0.154
-
ATP
-
Homo sapiens
2.17
-
Gln
-
Homo sapiens
64.2
-
NH4+
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Homo sapiens
K+ Km: 8.32 mM Homo sapiens
Mg2+ required Homo sapiens
Mg2+ Km: 1.36 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
483000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.017
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
4Ā°C, 24 h, complete loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-Gln
-
Homo sapiens AMP + diphosphate + NAD+ + L-Glu
-
?
ATP + deamido-NAD+ + NH4+
-
Homo sapiens AMP + diphosphate + NAD+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.4
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 6.0: about 75% of maximal activity, 8.0: about 55% of maximal activity Homo sapiens