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Literature summary for 6.3.5.1 extracted from

  • Rising, K.A.; Schramm, C.L.
    Enzymatic synthesis of NAD+ with the specific incorporation of atomic labels (1994), J. Am. Chem. Soc., 116, 6531-6536.
No PubMed abstract available

Application

Application Comment Organism
synthesis enzymatic method for efficient synthesis of NAD+ of high purity with 3H, 14C, or other labels at any nonexchangeable position of the NMN+ or AMP portions of the NAD+ molecule Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000 + x * 80000, SDS-PAGE Saccharomyces cerevisiae
80000
-
x * 65000 + x * 80000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.225
-
-
Saccharomyces cerevisiae
2.1
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-Gln
-
Saccharomyces cerevisiae AMP + diphosphate + NAD+ + L-Glu
-
?

Subunits

Subunits Comment Organism
? x * 65000 + x * 80000, SDS-PAGE Saccharomyces cerevisiae