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Literature summary for 6.3.4.6 extracted from

  • Roon, R.J.; Levenberg, B.
    Urea amidolyase. I. Properties of the enzyme from Candida utilis (1972), J. Biol. Chem., 247, 4107-4113.
    View publication on PubMed
Search for more literature for 6.3.4.6

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Cyberlindnera jadinii
Avidin
-
 Chlamydomonas reinhardtii
Avidin
-
 Cyberlindnera jadinii
Avidin
-
 Saccharomyces cerevisiae
BaCl2
-
 Cyberlindnera jadinii
CaCl2
-
 Cyberlindnera jadinii
CoCl2
-
 Cyberlindnera jadinii
F-
-
 Cyberlindnera jadinii
HgCl2
-
 Cyberlindnera jadinii
iodoacetate
-
 Cyberlindnera jadinii
PCMB
-
 Cyberlindnera jadinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Urea
-
 Cyberlindnera jadinii
0.1
-
 Urea
-
 Saccharomyces cerevisiae
0.25
-
 ATP
-
 Cyberlindnera jadinii
0.5
-
 HCO3-
-
 Cyberlindnera jadinii

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ monovalent cation required, K+, Rb+, Cs+, or NH4+ Cyberlindnera jadinii
K+ monovalent cation required, K+, Rb+, Cs+ or NH4+ Cyberlindnera jadinii
Mg2+ divalent cation required, Mg2+ or Mn2+, maximal activity at 8 mM Cyberlindnera jadinii
Mn2+ divalent cation required, Mg2+ or Mn2+, maximal activity at 2 mM, much less effective than Mg2+ at the optimal level Cyberlindnera jadinii
NH4+ monovalent cation required, K+, Rb+, Cs+, or NH4+ Cyberlindnera jadinii
Rb+ monovalent cation required, K+, Rb+, Cs+, or NH4+ Cyberlindnera jadinii

Organism

Organism UniProt Comment Textmining
Auxenochlorella pyrenoidosa
-
 grown on urea as sole nitogen source
-
Chlamydomonas reinhardtii
-
 grown on urea as sole nitrogen source
-
Cyberlindnera jadinii
-
-
-
Cyberlindnera jadinii
-
 inducible enzyme
-
Debaryomyces subglobosus
-
 grown on urea as sole nitrogen source
-
Pseudochlorella pringsheimii
-
 grown on urea as sole nitogen source
-
Saccharomyces cerevisiae
-
 grown on urea as sole nitrogen source
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
ATP + acetamide + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + cyanamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
ATP + formamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
ATP + formamide + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + hydroxyurea + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
ATP + hydroxyurea + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + imidodicarbonic acid diamide + HCO3- iminodicarbonic acid diamide, i.e. biuret Cyberlindnera jadinii ?
-
 ?
ATP + N-methylurea + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
ATP + N-methylurea + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + propionamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
ATP + urea + HCO3-
-
 Chlamydomonas reinhardtii ADP + phosphate + allophanate
-
 ?
ATP + urea + HCO3-
-
 Cyberlindnera jadinii ADP + phosphate + allophanate
-
 ?
ATP + urea + HCO3-
-
 Saccharomyces cerevisiae ADP + phosphate + allophanate
-
 ?
ATP + urea + HCO3-
-
 Pseudochlorella pringsheimii ADP + phosphate + allophanate
-
 ?
ATP + urea + HCO3-
-
 Auxenochlorella pyrenoidosa ADP + phosphate + allophanate
-
 ?
ATP + urea + HCO3-
-
 Debaryomyces subglobosus ADP + phosphate + allophanate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable for short periods of time Cyberlindnera jadinii
45
-
 10 min, 50% loss of activity Cyberlindnera jadinii
55
-
 10 min, complete denaturation Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 7.9
-
 Cyberlindnera jadinii

pH Range

pH Minimum pH Maximum Comment Organism
7.3 8.7 about 50% of maximal activity at pH 7.3 and 8.7 Cyberlindnera jadinii